A monotetrazolium indicator dye
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Iodonitrotetrazolium (chloride)

Item No. 16073

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium, monochloride
CAS Number
146-68-9
Synonyms
  • INT
  • Iodonitrotetrazolum Violet
  • NSC 27620
Molecular Formula
C19H13IN5O2 • Cl
Formula Weight
505.7
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMSO: 0.5 mg/mlDMSO:PBS(pH7.2) (1:1): 0.5 mg/mlEthanol: 30 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
249 nm
SMILES
IC(C=C1)=CC=C1[N+]2=NC(C3=CC=CC=C3)=NN2C4=CC=C([N+]([O-])=O)C=C4.[Cl-]
InChi Code
InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1
InChi Key
JORABGDXCIBAFL-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

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    1 g

    $41

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    5 g

    $186

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    10 g

    $351

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    25 g

    $823

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    Product Description

    Iodonitrotetrazolium (INT) is a monotetrazolium salt used as an indicator dye.1,2 It is reduced to an insoluble formazan that is used as a vital dye or indicator of cellular redox activity.1 Reduction commonly results from the activity of dehydrogenases, although non-enzymatic electron transfer reactions can occur in the presence of an intermediate electron acceptor.1,3 INT is commonly used to measure the respiratory activity of microorganisms in a variety of contexts.1,2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol. Ann. Rev. 11, 127-152 (2005).

    2. Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010).

    3. Green, J.D., and Narahara, H.T. Assay of succinate dehydrogenase activity by the tetrazolium method: Evaluation of an improved technique in skeletal muscle fractions. J. Histochem. Cytochem. 28(5), 408-412 (1980).