For immunochemical detection of AIF
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AIF Polyclonal Antibody

Item No. 160773

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Apoptosis-Inducing Factor
  • Programmed Cell Death Protein 8
Immunogen
Synthetic peptide from an internal region of human AIF
Formulation (Request formulation change)
500 µl of peptide affinity purified polyclonal antibody
Storage Buffer
TBS, pH 7.4 with 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide
Host
Rabbit
Applications
WB
Species Reactivity
(+) Human AIF(+) Mouse AIF(+) Rat AIF
UniProt Accession №
O95831
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    1 ea (500 µl)

    $347

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    Product Description

    Apoptosis-inducing factor (AIF) is a highly conserved mitochondrial protein with roles in redox-biochemistry and apoptosis.1,2 Apoptosis is a controlled process of cell death necessary for proper physiological development and maintenance. Loss of mitochondrial membrane potential results in the release of several proteins critical to the acceleration of apoptosis.3 When AIF is released from the mitochondrial intermembrane space it migrates to the nucleus to initiate chromatin condensation and DNA cleavage.4,5,6 AIF is recognized by immunoblotting at 67 kDa in most tissues and cell lines.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lorenzo, H.K., Susin, S.A., Penninger, J., et al. Apoptosis inducing factor (AIF): A phylogenetically old, caspase-independent effector of cell death. Cell Death Differ. 6, 516-524 (1999).

    2. Joza, N., Susin, S.A., Daugas, E., et al. Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death. Nature 410, 549-554 (2001).

    3. Green, D.R., and Reed, J.C. Mitochondria and apoptosis. Science 281, 1309-1312 (1998).

    4. Susin, S.A., Lorenzo, H.K., Zamzami, N., et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 397, 441-446 (1999).

    5. Susin, S.A., Zamzami, N., Castedo, M., et al. Bcl-2 inhibits the mitochondrial release of an apoptogenic protease. J. Exp. Med. 184, 1331-1341 (1996).

    6. Daugas, E., Susin, S.A., Zamzami, N., et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. The FASEB Journal 14, 729-739 (2000).