For immunochemical detection of Nod2
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Nod2 Polyclonal Antibody

Item No. 160777

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Caspase recruitment domain-containing protein 15
  • Inflammatory bowel disease protein 1
  • Nucleotide-binding oligomerization domain-containing protein 2
Immunogen
Recombinant protein fragment from the internal region of human Nod2
Formulation (Request formulation change)
100 μg of protein A purified antibody
Storage Buffer
TBS, pH 7.4, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide
Host
Rabbit
Applications
WB
Species Reactivity
(+) Human
UniProt Accession №
Q9HC29
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    Nod2 is a member of a protein family of apoptosis regulators which includes Apaf-1 and Nod1/CARD4.1 Nod2 contains two N-terminal caspase recruitment domains (CARDs), a nucleotide binding domain (NBD) and C-terminal leucine rich repeats (LRRs). Nod1/CARD4 is broadly expressed in tissues; however, Nod2 expression is limited to monocytes.1,2 Both Nod1 and Nod2 activate NF-kB and act as intracellular receptors for bacterial lipopolysaccharides (LPS).2,3,4,5 The LRRs of both Nod1 and Nod2 are essential for LPS-induced activation of NF-kB.4 A frameshift mutation in the NOD2 gene confers susceptibility to Crohn’s disease, possibly by causing truncation of the 10th LRR resulting in a protein that is unresponsive to LPS.6,7

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Ogura, Y., Inohara, N., Benito, A., et al. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-κB. The Journal of Biological Chemisty 276(7), 4812-4818 (2001).

    2. Bertin, J., Nir, W.J., Fischer, C.M., et al. Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-κB. The Journal of Biological Chemisty 274(19), 12955-12958 (1999).

    3. Inohara, N., Koseki, T., Lin, J., et al. An induced proximity model for NF-κB activation in the Nod1/RICK and RIP signaling pathways. J. Biochem. 275(36), 27823-27831 (2000).

    4. Inohara, N., Ogura, Y., Chen, F.F., et al. Human NOD1 confers responsiveness to bacterial lipopolysaccharides. The Journal of Biological Chemisty 276(4), 2551-2554 (2001).

    5. Inohara, N., Koseki, T., del Peso, L., et al. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-κB. The Journal of Biological Chemisty 274(21), 14560-14567 (1999).

    6. Ogura, Y., Bonen, D.K., Inohara, N., et al. A frameshift mutation in NOD2 associated with susceptibility to Crohn’s disease. Nature 411, 603-606 (2001).

    7. Hugot, J.P., Chamaillard, M., Zouali, H., et al. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn’s disease. Nature 411, 599-603 (2001).