For immunochemical detection of nNOS
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nNOS Polyclonal Antibody

Item No. 160870

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Neuronal Nitric Oxide Synthase
  • NOS I
  • ncNOS
Immunogen
Synthetic peptide from the C-terminal region of human nNOS
Formulation (Request formulation change)
500 µl of Peptide affinity-purified polyclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
ICC, IHC, and WB
Cross Reactivity
(−) eNOS(−) iNOS
Species Reactivity
(+) Human(+) Rat
UniProt Accession №
P29475
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea (500 µl)

    $347

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    Product Description

    Nitric oxide synthase (NOS) catalyzes the oxidation of arginine to nitric oxide (NO) and citrulline. Three distinct isoforms of NOS have been described having nomenclature based on the tissue source from which they were originally cloned. These three isoforms are neuronal/brain NOS (nNOS/bNOS/NOS-I), inducible NOS (iNOS/NOS-II), and endothelial NOS (eNOS/NOS-III).1,2 nNOS is a soluble enzyme found in brain, the peripheral nervous system and skeletal muscle.3,4 An alternately spliced form of nNOS (nNOSμ) containing a 34 amino acid insert has been identified in skeletal muscle.5 In neurons, protein-protein interactions with PSD95 and PSD93 via the PZD domain at the N-terminus of nNOS localizes the enzyme with NMDA receptors.6,7 Although nNOS was originally thought to be constitutively expressed, abundant evidence suggests expression is regulated by a variety of conditions.8

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Michel, T., Xie, Q.-W., and Nathan, C. Molecular biological analysis of nitric oxide synthases. Methods in nitric oxide research 161-175 (1996).

    2. Michel, T., and Feron, O. Nitric oxide synthases: Which, where, how, and why? J. Clin. Invest. 100(9), 2146-2152 (1997).

    3. Bredt, D.S., Hwang, P.M., Glatt, C.E., et al. Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase. Nature 351(6329), 714-718 (1991).

    4. Nakane, M., Schmidt, H.H.W., Pollock, J.S., et al. Cloned human brain nitric oxide synthase is highly expressed in skeletal muscle. FEBS Lett. 316(2), 175-180 (1993).

    5. Silvagno, F., Xia, H., and Bredt, D.S. Neuronal nitric-oxide synthase-μ, an alternatively spliced isoform expressed in differentiated skeletal muscle. The Journal of Biological Chemisty 271(19), 11204-11208 (1996).

    6. Brenman, J.E., Christopherson, K.S., Craven, S.E., et al. Cloning and characterization of postsynaptic density 93, a nitric oxide synthase interacting protein. J. Neurosci. 16(23), 7407-7415 (1996).

    7. Christopherson, K.S., and Bredt, D.S. Nitric oxide in excitable tissues: Physiological roles and disease. J. Clin. Invest. 100(10), 2424-2429 (1997).

    8. Wang, Y., Newton, D.C., and Marsden, P.A. Neuronal NOS: Gene structure, mRNA diversity, and functional relevance. Crit. Rev. Neurobiol. 13(1), 21-43 (1999).

    Product Citations

    Greco, R., Ferrigno, A., Demartini, C., et al. Evaluation of ADMA-DDAH-NOS axis in specific brain areas following nitroglycerin administration: Study in an animal model of migraine. J. Headache Pain 16(74), (2015).

    Carney, S.T., Lloyd, M.L., MacKinnon, S.E., et al. Cannabinoid regulation of nitric oxide synthase I (nNOS) in neuronal cells. J. Neuroimmune Pharmacol. 4(3), 338-349 (2009).