A fluorogenic substrate for lysosomal acid lipase
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4-Methylumbelliferyl Palmitate

Item No. 16089

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
hexadecanoic acid, 4-methyl-2-oxo-2H-1-benzopyran-7-yl ester
CAS Number
17695-48-6
Synonyms
  • 4-MUP
  • 4-MU Palmitate
  • Palmitoyl 4-Methylumbelliferone
Molecular Formula
C26H38O4
Formula Weight
414.6
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
Chloroform: 20 mg/mlDMF: 10 mg/mlDMSO: 0.1 mg/ml
λmax
274, 310 nm
SMILES
O=C1C=C(C)C2=C(C=C(OC(CCCCCCCCCCCCCCC)=O)C=C2)O1
InChi Code
InChI=1S/C26H38O4/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-25(27)29-22-17-18-23-21(2)19-26(28)30-24(23)20-22/h17-20H,3-16H2,1-2H3
InChi Key
QKQVFFACWLFRRX-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    100 mg

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    Product Description

    Cholesterol ester storage disease and Wolman disease are recessive autosomal disorders caused by a deficiency in lysosomal acid lipase (LAL), also known as cholesteryl ester hydrolase.1,2 4-Methylumbelliferyl palmitate (4-MUP) is a fluorogenic substrate for lysosomal acid lypase (LAL).3 4-MUP is cleaved by LAL to release the fluorescent moiety 4-MU. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases.4 4-MUP may also be cleaved by other acid lipases.5,6 Recent advances allow the assessment of LAL activity in very small blood volumes using 4-MUP.1,2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Dairaku, T., Iwamoto, T., Nishimura, M., et al. A practical fluorometric assay method to measure lysosomal acid lipase activity in dried blood spots for the screening of cholesteryl ester storage disease and Wolman disease. Mol. Genet. Metab. 111(2), 193-196 (2014).

    2. Hamilton, J., Jones, I., Srivastava, R., et al. A new method for the measurement of lysosomal acid lipase in dried blood spots using the inhibitor Lalistat 2. Clin. Chim. Acta 413(15-16), 1207-1210 (2012).

    3. Kelly, S., and Bakhru-Kishore, R. Fluorimetric assay of acid lipase in human leukocytes. Clin. Chim. Acta 97(2-3), 239-242 (1979).

    4. Zhi, H., Wang, J., Wang, S., et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule. J. Spectrosc. 147128 (2013).

    5. Warner, T.G., Dambach, L.M., Shim, J.H., et al. Separation and characterization of the acid lipase and neutral esterases from human liver. Am. J. Hum. Genet. 32(6), 869-879 (1980).

    6. Baillargeon, M.W., and McCarthy, S.G. Geotrichum candidum NRRL Y-553 lipase: Purification, characterization and fatty acid specificity. Lipids 26(10), 831-836 (1991).