For immunochemical detection of sGC α1 subunit
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Guanylate Cyclase α subunit (soluble) Polyclonal Antibody

Item No. 160895

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • GUCY1A1
  • sGC α1 subunit
Immunogen
Peptide from the internal region of human protein
Formulation (Request formulation change)
500 µl of Peptide affinity-purified polyclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
WB and IHC
Species Reactivity
(+) Human(+) Bovine(+) Mouse
UniProt Accession №
Q02108
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea (500 µl)

    $347

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    Product Description

    Soluble guanylate cyclase is a heterodimeric enzyme, composed of α and β subunits, that synthesizes cGMP from GTP. The enzyme is activated by the binding of nitric oxide or carbon monoxide to the heme group of the enzyme.1 Chronic hypoxia upregulates soluble guanylate expression in rat lung.2 The α1 subunit contains 690-717 amino acids and has a molecular mass of 77-82 kDa.3,4,5 The cloned β1 subunit of guanylate cyclase from human, bovine, and rat sources contains 619 amino acids and has a molecular mass of approximately 70,000.3,6,7

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Stone, J.R., and Marletta, M.A. Soluble guanylate cyclase from bovine lung: Activation with nitric oxide and carbon monoxide and spectral characterization of the ferrous and ferric states. Biochemistry 33(18), 5636-5640 (1994).

    2. Li, D., Zhou, N., and Johns, R.A. Soluble guanylate cyclase gene expression and localization in rat lung after exposure to hypoxia. Am. J. Physiol. 277(4), 841-847 (1999).

    3. Giuili, G., Scholl, U., Bulle, F., et al. Molecular cloning of the cDNAs coding for the two subunits of soluble guanylyl cyclase from human brain. FEBS Lett. 304(1), 83-88 (1992).

    4. Nakane, M., Arai, K., Saheki, S., et al. Molecular cloning and expression of cDNAs coding for soluble guanylate cyclase from rat lung. The Journal of Biological Chemisty 265(28), 16841-16845 (1990).

    5. Lee, M.H., and Bell, R.M. Supplementation of the phosphatidyl-L-serine requirement of protein kinase C with nonactivating phospholipids. Biochemistry 31(22), 5176-5182 (1992).

    6. Koesling, D., Herz, J., Gausepohl, H., et al. The primary structure of the 70 kDa subunit of bovine soluble guanylate cyclase. FEBS Lett. 239(1), 29-34 (1988).

    7. Nakane, M., Saheki, S., Kuno, T., et al. Molecular cloning of a cDNA coding for 70 kilodalton subunit of soluble guanylate cyclase from rat lung. Biochem. Biophys. Res. Commun. 157(3), 1139-1147 (1988).