An ecdysteroid hormone
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

20-hydroxy Ecdysone

Item No. 16145

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(5β)-2β,3β,14,20,22R,25-hexahydroxy-cholest-7-en-6-one
CAS Number
5289-74-7
Synonyms
  • Ecdysterone
  • Isoinokosterone
Molecular Formula
C27H44O7
Formula Weight
480.6
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlEthanol: 25 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
243 nm
SMILES
O=C1[C@]2([H])C[C@@H](O)[C@@H](O)C[C@]2(C)[C@]3([H])C([C@@](CC[C@]4([H])[C@]([C@H](O)CCC(C)(O)C)(O)C)(O)[C@]4(C)CC3)=C1
InChi Code
InChI=1S/C27H44O7/c1-23(2,32)9-8-22(31)26(5,33)21-7-11-27(34)16-12-18(28)17-13-19(29)20(30)14-24(17,3)15(16)6-10-25(21,27)4/h12,15,17,19-22,29-34H,6-11,13-14H2,1-5H3/t15-,17-,19+,20-,21-,22+,24+,25+,26+,27+/m0/s1
InChi Key
NKDFYOWSKOHCCO-YPVLXUMRSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    5 mg

    $54

    Add

    10 mg

    $96

    Add

    25 mg

    $158

    Add

    50 mg

    $290

    Add
    Lipid Resource Center
    Discover Products & Resources for Lipid Research
    • High-purity lipid standards
    • Lipid roles in biology
    • Lipids in health & disease
    • Lipids for pharmaceutical development
    • Protocols, advice, & resources
    EXPLORE NOW
    Product Description

    20-hydroxy Ecdysone is an ecdysteroid hormone produced in arthropod species that induces developmental changes associated with ecdysis and the completion of metamorphosis.1 It is also produced in plants as a defensive strategy to disrupt the development of insect pests.2 In target tissues, 20-hydroxy ecdysone binds to the nuclear hormone receptor, ecdysone receptor, initiating a signaling cascade that results in cell cycle arrest.1,3 Ecdysteroids such as this compound have been employed as tools in inducible gene regulation systems controlled by reporter cells transfected with the ecdysone receptor.4,5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Fallon, A.M., and Gerenday, A. Ecdysone and the cell cycle: Investigations in a mosquito cell line. J. Insect Physiol. 56(10), 1396-1401 (2010).

    2. Boo, K.H., Lee, D., Jeon, G.L., et al. Distribution and biosynthesis of 20-hydroxyecdysone in plants of Achyranthes japonica Nakai. Biosci. Biotechnol. Biochem. 74(11), 2226-2231 (2010).

    3. Cai, M.J., Dong, D.J., Wang, Y., et al. G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane. Cell Commun. Signal. 12, 11-16 (2014).

    4. Saez, E., Nelson, M.C., Eshelman, B., et al. Identification of ligands and coligands for the ecdysone-regulated gene switch. Proc. Natl. Acad. Sci. USA 97(26), 14512-14517 (2000).

    5. Karzenowski, D., Potter, D.W., and Padidam, M. Inducible control of transgene expression with ecdysone receptor: Gene switches with high sensitivity, robust expression, and reduced size. Biotechniques 39(2), 191-192 (2005).