A fluorescent calcium probe
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Calcein

Item No. 16221

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N,N'-[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl)bis(methylene)]bis[N-(carboxymethyl)-glycine
CAS Number
1461-15-0
Synonyms
  • Fluorescein Complexone
  • NSC 298193
Molecular Formula
C30H26N2O13
Formula Weight
622.5
Emission
517 nm
Excitation
494 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
1 M NaOH: 50 mg/mlPBS (pH 7.2): 0.2 mg/ml
λmax
226, 278 nm
SMILES
OC1=CC2=C(C=C1CN(CC(O)=O)CC(O)=O)C3(C(C=CC=C4)=C4C(O3)=O)C5=CC(CN(CC(O)=O)CC(O)=O)=C(O)C=C5O2
InChi Code
InChI=1S/C30H26N2O13/c33-21-7-23-19(5-15(21)9-31(11-25(35)36)12-26(37)38)30(18-4-2-1-3-17(18)29(43)45-30)20-6-16(22(34)8-24(20)44-23)10-32(13-27(39)40)14-28(41)42/h1-8,33-34H,9-14H2,(H,35,36)(H,37,38)(H,39,40)(H,41,42)
InChi Key
DEGAKNSWVGKMLS-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

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    1 g

    $36

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    5 g

    $62

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    10 g

    $103

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    25 g

    $186

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    Product Description

    Calcein is a xanthene that is commonly used for the fluorometric determination of calcium in solution.1,2 As this form of calcein is not membrane permeable, it can be used in assays that evaluate membrane integrity.3,4 Calcein can also be used as a fluorescent indicator for fluoride, iron, and mercury.5,6,7 Excitation and emission maxima for calcein are 494 and 517 nm, respectively.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bandrowski, J.F., and Benson, C.L. Investigation of the use of calcein in the ultramicro fluorometric determination of calcium in serum. Clin. Chem. 18(11), 1411-1414 (1972).

    2. Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010).

    3. Cutler, A.J., and Saleem, M. Permeabilizing soybean protoplasts to macromolecules using electroporation and hypotonic shock. Plant Physiol. 83(1), 24-28 (1987).

    4. Kendall, D.A., and MacDonald, R.C. A fluorescence assay to monitor vesicle fusion and lysis. The Journal of Biological Chemisty 257(23), 13892-13895 (1982).

    5. Li, H.B., and Chen, F. A highly sensitive fluorimetric method for the determination of fluoride in biological material with Al3+-calcein complex. Fresenius. J. Anal. Chem. 368(5), 501-504 (2000).

    6. Ali, A., Zhang, Q., Dai, J., et al. Calcein as a fluorescent iron chemosensor for the determination of low molecular weight iron in biological fluids. BioMetals 16(2), 285-293 (2003).

    7. Lista, A.G., Palomeque, M.E., and Fernández Band, B.S. Flow-injection fluorimetric determination of mercury(II) with calcein. Talanta 50(4), 881-885 (1999).