A cell-permeable inhibitor of Ape-1
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DNA Base Excision Repair Pathway Inhibitor

Item No. 16348

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-nitro-1H-indole-2-carboxylic acid
CAS Number
6960-45-8
Synonyms
  • CRT0044876
  • 7-Nitroindole-2-Carboxylic Acid
  • NSC 69877
Molecular Formula
C9H6N2O4
Formula Weight
206.2
Purity
≥98%
Formulation
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 5 mg/mlDMSO:PBS(pH7.2) (1:1): 0.5 mg/mlEthanol: 0.2 mg/ml
λmax
222, 243, 358 nm
SMILES
OC(C1=CC2=CC=CC([N+]([O-])=O)=C2N1)=O
InChi Code
InChI=1S/C9H6N2O4/c12-9(13)6-4-5-2-1-3-7(11(14)15)8(5)10-6/h1-4,10H,(H,12,13)
InChi Key
BIUCOFQROHIAEO-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    10 mg

    $49

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    50 mg

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    100 mg

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    Cayman Chemical
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    Product Description

    Apurinic/apyrimidinic endonuclease-1 (Ape-1) is involved in base excision repair, cleaving the phosphodiester linkage that is 5’ to the abasic site to produce a single strand break.1 DNA base excision repair pathway inhibitor is a cell-permeable inhibitor of Ape-1 (IC50 = 3-11 µM).1,2,3 Although not toxic to cells by itself at 200 µM, it enhances the cytotoxicity of certain DNA alkylating agents, including temozolomide, when added at this concentration.2 DNA base excision repair pathway inhibitor has little or no effect on radiation-induced cell survival.2,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Srinivasan, A., Wang, L., Cline, C.J., et al. Identification and characterization of human apurinic/apyrimidinic endonuclease-1 inhibitors. Biochemistry 51(31), 6246-6259 (2012).

    2. Madhusudan, S., Smart, F., Shrimpton, P., et al. Isolation of a small molecule inhibitor of DNA base excision repair. Nucleic Acids Res. 33(15), 4711-4724 (2005).

    3. Mohammed, M.Z., Vyjayanti, V.N., Laughton, C.A., et al. Development and evaluation of human AP endonuclease inhibitors in melanoma and glioma cell lines. Br. J. Cancer 104(4), 653-663 (2011).

    4. Naidu, M.D., Mason, J.M., Pica, R.V., et al. Radiation resistance in glioma cells determined by DNA damage repair activity of Ape1/Ref-1. J. Radiat. Res. 51(4), 393-404 (2010).