A chromophoric substrate for measuring inorganic phosphate
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7-methyl-6-Thioguanosine (technical grade)

Item No. 16381

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-amino-6,9-dihydro-7-methyl-9-β-D-ribofuranosyl-6-thioxo-1H-purinium, inner salt
CAS Number
55727-10-1
Synonyms
  • MESG
Molecular Formula
C11H15N5O4S
Formula Weight
313.3
Purity
≥85%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 50 mg/mlDMSO: 30 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
212, 263, 345 nm
SMILES
O[C@H]1[C@@H](O)[C@H](N2C=[N+](C)C3=C2N=C([NH-])NC3=S)O[C@@H]1CO
InChi Code
InChI=1S/C11H15N5O4S/c1-15-3-16(8-5(15)9(21)14-11(12)13-8)10-7(19)6(18)4(2-17)20-10/h3-4,6-7,10,17-19H,2H2,1H3,(H2-,12,13,14,21)/t4-,6-,7-,10-/m1/s1
InChi Key
RFHIWBUKNJIBSE-KQYNXXCUSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $116

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    10 mg

    $215

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    25 mg

    $505

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    Product Description

    7-methyl-6-Thioguanosine is a chromophoric substrate that is used to quantify inorganic phosphate (Pi). In the presence of purine nucleoside phosphorylase, it reacts with Pi to produce 7-methyl-6-thioguanine and ribose 1-phosphate, with the increase in 7-methyl-6-thioguanine followed by continuous spectrophotometry at an absorbance of 355-360 nm.1 7-methyl-6-Thioguanosine is also used to measure changes in Pi levels in coupled enzyme systems, including assays of the activities of protein phosphatases, phosphorylase kinases, GTPases, and ATPases.1,2,3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Webb, M.R. A continuous spectrophotometric assay for inorganic phosphate and for measuring phosphate release kinetics in biological systems. Proc. Natl. Acad. Sci. USA 89(11), 4884-4887 (1992).

    2. Cheng, Q., Wang, Z.X., and Killilea, S.D. A continuous spectrophotometric assay for protein phosphatases. Anal. Biochem. 310(2), 156-162 (2002).

    3. Wang, Z.X., Cheng, Q., and Killilea, S.D. A continuous spectrophotometric assay for phosphorylase kinase. Anal. Biochem. 230(1), 55-61 (1995).

    4. Tan, Y.C., Wu, W.N., Zheng, Y., et al. Characterization of the interactions between the small GTPase RhoA and its guanine nucleotide exchange factors. Anal. Biochem. 310(2), 156-162 (2014).