A fluorogenic substrate for phosphatases
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4-Methylumbelliferyl Phosphate

Item No. 16645

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-methyl-7-(phosphonooxy)-2H-1-benzopyran-2-one
CAS Number
3368-04-5
Synonyms
  • 7-hydroxy-4-Methylcoumarin Phosphate
  • 4-Methylumbelliferone Phosphate
  • 4-MUP
  • 4-MU Phosphate
Molecular Formula
C10H9O6P
Formula Weight
256.2
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 10 mg/mlDMSO: 20 mg/mlPBS (pH 7.2): 5 mg/ml
λmax
285, 315 nm
SMILES
O=C1C=C(C)C2=C(C=C(OP(O)(O)=O)C=C2)O1
InChi Code
InChI=1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
InChi Key
BCHIXGBGRHLSBE-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

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    250 mg

    $32

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    500 mg

    $60

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    1 g

    $107

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    Product Description

    4-Methylumbelliferyl Phosphate (4-MUP) is a fluorogenic substrate for phosphatases, including acid and alkaline phosphatases.1,2,3 It is converted to the fluorescent product 4-methylumbelliferone (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Fernley, H.N., and Walker, P.G. Kinetic behaviour of calf-intestinal alkaline phosphatase with 4-methylumbelliferyl phosphate. Biochem. J. 97(1), 95-103 (1965).

    2. Kohli, A.G., Kieler-Ferguson, H.M., Chan, D., et al. A robust and quantitative method for tracking liposome contents after intravenous administration. J. Control Release 176, 86-93 (2014).

    3. Remaley, A.T., Kuhn, D.B., Basford, R.E., et al. Leishmanial phosphatase blocks neutrophil O2 production. The Journal of Biological Chemisty 259(18), 11173-11175 (1984).

    4. Zhi, H., Wang, J., Wang, S., et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule. J. Spectrosc. 147128 (2013).