A fluorescent pH probe
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

BCECF

Item No. 17183

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5(or 6)-carboxy-3’,6’-dihydroxy-3-oxo-spiro[isobenzofuran-1(3H),9’-[9H]xanthene]-2’,7’-dipropanoic acid
CAS Number
85138-49-4
Synonyms
  • 2',7'-bis(carboxyethyl)-5(6)-Carboxyfluorescein
Molecular Formula
C27H20O11
Formula Weight
520.4
Purity
≥90%
Emission
535 nm
Excitation
440, 490 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 5 mg/mlDMSO: 15 mg/mlDMSO:PBS (pH 7.2) (1:2): 0.25 mg/mlEthanol: 5 mg/ml
λmax
210, 228, 280, 460, 491 nm
SMILES
OC1=C(CCC(O)=O)C=C(C2(C(C=CC(C(O)=O)=C3)=C3C(O2)=O)C(C=C(CCC(O)=O)C(O)=C4)=C4O5)C5=C1.OC6=C(CCC(O)=O)C=C(C7(C(C=C(C(O)=O)C=C8)=C8C(O7)=O)C(C=C(CCC(O)=O)C(O)=C9)=C9O%10)C%10=C6
InChi Code
InChI=1S/2C27H20O11/c28-19-10-21-17(8-12(19)2-5-23(30)31)27(16-4-1-14(25(34)35)7-15(16)26(36)38-27)18-9-13(3-6-24(32)33)20(29)11-22(18)37-21;28-19-10-21-17(7-12(19)2-5-23(30)31)27(16-9-14(25(34)35)1-4-15(16)26(36)38-27)18-8-13(3-6-24(32)33)20(29)11-2
InChi Key
RZFSVSQVNQXHLI-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    500 µg

    $41

    Add

    1 mg

    $72

    Add

    5 mg

    $255

    Add
    Product Description

    BCECF is a fluorescent probe commonly used to measure pH.1 It is a dual-excitation ratiometric pH indicator with a pKa of ~6.98. Measurements of pH are made by determining the ratio of emission intensity, detected at 535 nm, when excited at 490 nm versus the emission intensity when excited at 440 nm.1 BCECF is membrane impermeable, whereas the acetoxymethyl ester, BCEBF-AM (Item No. 15922) is membrane permeant.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. O'Connor, N., and Silver, R.B. Ratio imaging: Practical considerations for measuring intracellular Ca2+ and pH in living cells. Methods Cell Biol. 81, 415-433 (2007).