A fluorogenic substrate
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4-Methylumbelliferyl-β-D-Glucuronide (hydrate)

Item No. 17203

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-methyl-2-oxo-2H-1-benzopyran-7-yl-β-D-glucopyranosiduronic acid, dihydrate
Synonyms
  • 4-Methylumbelliferyl-β-D-Glucopyranosiduronic Acid
  • MUG
Molecular Formula
C16H16O9 • 2H2O
Formula Weight
388.3
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMSO: 5 mg/mlEthanol: 2 mg/mlPBS (pH 7.2): 2 mg/ml
λmax
319 nm
SMILES
O[C@H]1[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](C(O)=O)[C@@H](O)[C@@H]1O.O.O
InChi Code
InChI=1S/C16H16O9.2H2O/c1-6-4-10(17)24-9-5-7(2-3-8(6)9)23-16-13(20)11(18)12(19)14(25-16)15(21)22;;/h2-5,11-14,16,18-20H,1H3,(H,21,22);2*1H2/t11-,12-,13+,14-,16+;;/m0../s1
InChi Key
WRVAYRGWFCRSBR-CMNHCFHDSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    250 mg

    $36

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    500 mg

    $68

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    1 g

    $117

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    5 g

    $502

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    Product Description

    4-Methylumbelliferyl-β-D-glucuronide is a fluorogenic substrate of β-glucuronidase.1 4-Methylumbelliferyl-β-D-glucuronide is cleaved by β-glucuronidase to release the fluorescent moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. 4-Methylumbelliferyl-β-D-glucuronide has been used in the β-glucuronidase reporter system, also known as the GUS reporter system, for fluorescent detection of β-glucuronidase gene expression in E. coli and transformed plants.1,2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Jefferson, R.A., Kavanagh, T.A., and Bevan, M.W. GUS fusions: β-Glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6(13), 3901-3907 (1987).

    2. Moberg, L.J. Fluorogenic assay for rapid detection of Escherichia coli in food. Appl. Environ. Microbiol. 50(6), 1383-1387 (1985).