For selection of orotidine-5′-phosphate decarboxylase mutants of yeast
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Product Type

Biochemicals

5-fluoro Orotic Acid (hydrate)

Item No. 17318

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-fluoro-1,2,3,6-tetrahydro-2,6-dioxo-4-pyrimidinecarboxylic acid, monohydrate
CAS Number
220141-70-8
Synonyms
  • 5-FOA
  • 5-fluoro OA
Molecular Formula
C5H3FN2O4 • H2O
Formula Weight
192.1
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 5 mg/mlDMSO: 10 mg/mlDMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml
λmax
288 nm
SMILES
O=C1NC(C(O)=O)=C(F)C(N1)=O.O
InChi Code
InChI=1S/C5H3FN2O4.H2O/c6-1-2(4(10)11)7-5(12)8-3(1)9;/h(H,10,11)(H2,7,8,9,12);1H2
InChi Key
LODRRYMGPWQCTR-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    500 mg

    $68

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    1 g

    $124

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    Product Description

    5-fluoro Orotic acid (5-FOA) is used to detect URA3 gene expression in yeast molecular genetic constructs.1 Yeast with an active URA3 gene, which encodes orotidine-5'-monophosphate decarboxylase, converts 5-FOA to fluorodeoxyuridine, which is toxic to cells. Resistant strains of yeast that carry a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Winston, F., Chaleff, D.T., Valent, B., et al. Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2), 179-197 (1984).