A fluorescent pH probe
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3,6-diacetoxy Phthalonitrile

Item No. 17409

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3,6-bis(acetyloxy)-1,2-benzenedicarbonitrile
CAS Number
83619-73-2
Synonyms
  • ADB
  • 1,4-Diacetoxy-2,3-dicyanobenzene
  • 2,3-Dicyano-1,4-hydroquinone diacetate
Molecular Formula
C12H8N2O4
Formula Weight
244.2
Purity
≥98%
Emission
450-476 nm (pH 5.0-10.0)
Excitation
351 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlDMSO:PBS(pH7.2) (1:1): 0.5 mg/ml
λmax
210, 305 nm
SMILES
CC(OC1=CC=C(OC(C)=O)C(C#N)=C1C#N)=O
InChi Code
InChI=1S/C12H8N2O4/c1-7(15)17-11-3-4-12(18-8(2)16)10(6-14)9(11)5-13/h3-4H,1-2H3
InChi Key
VXDCGBVAZDYTFK-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $101

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    10 mg

    $181

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    25 mg

    $376

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    Product Description

    3,6-diacetoxy Phthalonitrile is a fluorescent probe for monitoring intracellular pH by flow cytometry in living cells.1 It rapidly crosses cell membranes and is cleaved by cytosolic esterases to form the fluorescent pH indicator 2,3-dicyano-hydroquinone (DCH), which is excited at UV wavelengths. DCH can be excited at 351 nm, with pH-dependent emission in the range of 450-476 nm (pH 5.0-10.0) using a potassium-based buffer.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Cook, J.A., and Fox, M.H. Intracellular pH measurements using flow cytometry with 1,4-diacetoxy-2,3-dicyanobenzene. Cytometry 9(5), 441-447 (1988).