A 5′-nucleotide phosphodiesterase substrate
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4-Nitrophenyl Phenylphosphonate

Item No. 17436

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
P-phenyl-phosphonic acid, mono(4-nitrophenyl) ester
CAS Number
57072-35-2
Synonyms
  • bis(4-Nitrophenyl) Phenylphosphonate
  • p-Nitrophenyl Phenylphosphonate
  • para-Nitrophenyl Phenylphosphonate
  • NPPP
Molecular Formula
C12H10NO5P
Formula Weight
279.2
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mLDMF:PBS(pH 7.2)(1:1): 0.5 mg/mLDMSO: 14 mg/mL
λmax
215, 272 nm
SMILES
O=P(OC1=CC=C([N+]([O-])=O)C=C1)(O)C2=CC=CC=C2
InChi Code
InChI=1S/C12H10NO5P/c14-13(15)10-6-8-11(9-7-10)18-19(16,17)12-4-2-1-3-5-12/h1-9H,(H,16,17)
InChi Key
NRGZTHQFAQCJCQ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

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    500 mg

    $103

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    1 g

    $132

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    Product Description

    4-Nitrophenyl phenylphosphonate is a substrate for 5’-nucleotide phosphodiesterases.1 It is a more preferable substrate to 5’-nucleotide phosphodiesterases than naturally occurring nucleotides or bis(4-nitrophenyl) phosphate because of its stability, ease of synthesis, and higher rate of hydrolysis under saturating conditions.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Moe, O.A., Jr., and Butler, L.G. The catalytic mechanism of bovine intestinal 5'-nucleotide phosphodiesterase. pH and inhibition studies. The Journal of Biological Chemisty 258(11), 6941-6946 (1983).

    2. Kelly, S.J., Dardinger, D.E., and Butler, L.G. Hydrolysis of phosphonate esters catalyzed by 5'-nucleotide phosphodiesterase. Biochemistry 14(22), 4983-4988 (1975).