A fluorescent probe for α-keto acids
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Product Categories

Product Type

Biochemicals
Labeling & Detection
Fluorescent Probes
Reactive Probes

Research Area

Application

Fluorescence

1,2-Diamino-4,5-dimethoxybenzene (hydrochloride)

Item No. 17644

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4,5-dimethoxy-1,2-benzenediamine, dihydrochloride
CAS Number
131076-14-7
Synonyms
  • DDB
Molecular Formula
C8H12N2O2 • 2HCl
Formula Weight
241.1
Purity
≥95%
Emission
448 nm
Excitation
361 nm
Formulation (Request formulation change)
A crystalline solid
λmax
240, 298 nm
SMILES
COC1=C(OC)C=C(N)C(N)=C1.Cl.Cl
InChi Code
InChI=1S/C8H12N2O2.2ClH/c1-11-7-3-5(9)6(10)4-8(7)12-2;;/h3-4H,9-10H2,1-2H3;2*1H
InChi Key
ORAAOAMEUMZGGU-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    50 mg

    $41

    Add

    100 mg

    $72

    Add

    250 mg

    $99

    Add
    Product Description

    1,2-Diamino-4,5-dimethoxybenzene is an aromatic diamine that reacts with aldehydes to produce highly fluorescent benzimidazole derivatives with excitation/emission spectra of 361/448 nm.1 It can be reacted with ketones and carbonyls, including stress compounds such as glyoxal and methylglyoxal, to form fluorescent adducts that can be separated and quantitated by reversed-phase high-performance liquid chromatography.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Nakamura, M., Hara, S., Yamaguchi, M., et al. 1,2-Diamino-4,5-methylenedioxybenzene as a highly sensitive fluorogenic reagent for α-keto acids. Chem. Pharm. Bull. (Tokyo) 35(2), 687-692 (1987).

    2. McLellan, A.C., Phillips, S.A., and Thornalley, P.J. The assay of methylglyoxal in biological systems by derivatization with 1,2-diamino-4,5-dimethoxybenzene. Anal. Biochem. 206(1), 17-23 (1992).