L-Cycloserine

L-Cycloserine is a potent inhibitor of serine palmitoyl transferase (SPT), the first enzyme in the sphingolipid synthesis pathway.¹ It inhibits bacterial SPT activity by 80% at a concentration of 25 µM, which is 100 times more potent than D-cycloserine (Item No. 22194). L-Cycloserine inhibits SPT activity up to 86% in a dose-dependent manner in microsomes prepared from mouse brain following administration of doses ranging from 25-200 mg/kg. It also inhibits SPT in rabbit aorta and several aminotransferases in vitro and in vivo in rat.^2,3 L-Cycloserine inhibits the growth of M. tuberculosis through branched chain aminotransferase inactivation, and it does so more rapidly and potently than D-cycloserine (MICs = 0.3 and 2.3 µg/ml, respectively).⁴

WARNING This product is not for human or veterinary use.

1. Sundaram, K.S., and Lev, M. Inhibition of sphingolipid synthesis by cycloserine in vitro and in vivo. J. Neurochem. 42(2), 577-581 (1984).

2. Williams, R.D., Sgoutas, D.S., Zaatari, G.S., et al. Inhibition of serine palmitoyltransferase activity in rabbit aorta by L-cycloserine. J. Lipid Res. 28(12), 1478-1481 (1987).

3. Wong, D.T., Fuller, R.W., and Molloy, B.B. Inhibition of amino acid transaminases by L-cycloserine. Adv. Enzyme Regul. 11, 139-154 (1973).

4. Amorim Franco, T.M., Favrot, L., Vergnolle, O., et al. Mechanism-based inhibition of the Mycobacterium tuberculosis branched-chain aminotransferase by D- and I-cycloserine. ACS Chem Biol. 12(5), 1235-1244 (2017).