For immunochemical detection of citrullinated histone H1.4
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Histone H1.4 (Citrullinated R53) Polyclonal Antibody

Item No. 18073

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Immunogen
Synthetic peptide from human Histone H1.4 citrullinated at R53
Formulation (Request formulation change)
500 µl of peptide affinity-purified polyclonal antibody
Storage Buffer
TBS, pH 7.4, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide
Host
Rabbit
Applications
WB
Cross Reactivity
(-) Non-citrullinated histone H1
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    1 ea (500 µl)

    $440

    Add
    Cayman Chemical
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    Product Description

    Citrullinated histone H1.4 is a citrullinated form of histone H1.4, a nuclear protein that interacts with linker DNA regions between nucleosomes to provide nucleosome core structure stabilization.1 Histone H1 subtypes are comprised of a short N-terminal domain, a central globular domain necessary for DNA interaction, and long C-terminal domain that determines subtype binding properties by its variability. Histone H1.4 is expressed in most somatic cell types and is essential for survival of T47D and MCF-7 breast cancer cells and MCF-10A breast epithelial cells.2,3,4 It is subject to citrullination by protein-arginine deiminase 4 (PAD4) at arginine 54, which corresponds to arginine 53 of the immunizing peptide for Cayman’s Histone H1.4 (Citrullinated R53) Polyclonal Antibody. Citrullination releases histone 1 from chromatin, leading to chromatin decondensation in mouse embryonic stem cells.5 Cayman’s Histone H1.4 (Citrullinated R53) Polyclonal Antibody can be used for Western blot applications. The antibody recognizes citrullinated histone H1, and not unmodified H1, at 35 kDa from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Roque, A., Ponte, I., and Suau, P. Interplay between histone H1 structure and function. Biochim. Biophys. Acta 1859(3), 444-454 (2016).

    2. Öberg, C., Izzo, A., Schneider, R., et al. Linker histone subtypes differ in their effect on nucleosomal spacing in vivo. J. Mol. Biol. 419(3-4), 183-197 (2012).

    3. Meergans, T., Albig, W., and Doenecke, D. Varied expression patterns of human H1 histone genes in different cell lines. DNA Cell Biol. 16(9), 1041-1049 (1997).

    4. Sancho, M., Diani, E., Beato, M., et al. Depletion of human histone H1 variants uncovers specific roles in gene expression and cell growth. PLoS Genet. 4(10), e1000227 (2008).

    5. Christophorou, M.A., Castelo-Branco, G., Halley-Stott, R.P., et al. Citrullination regulates pluripotency and histone H1 binding to chromatin. Nature 507(7490), 104-108 (2014).