Brominated UTP for labeling RNA
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5-Bromouridine 5'-triphosphate (sodium salt)

Item No. 18140

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-bromo-uridine 5'-(tetrahydrogen triphosphate), trisodium salt
Synonyms
  • 5-BrUTP
Molecular Formula
C9H11BrN2O15P3 • 3Na
Formula Weight
629.0
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
PBS (pH 7.2): 10 mg/ml
λmax
210, 280 nm
SMILES
O[C@@H]1[C@H](O)[C@@H](COP(OP(OP(O)([O-])=O)([O-])=O)([O-])=O)O[C@H]1N2C=C(Br)C(NC2=O)=O.[Na+].[Na+].[Na+]
InChi Code
InChI=1S/C9H14BrN2O15P3.3Na/c10-3-1-12(9(16)11-7(3)15)8-6(14)5(13)4(25-8)2-24-29(20,21)27-30(22,23)26-28(17,18)19;;;/h1,4-6,8,13-14H,2H2,(H,20,21)(H,22,23)(H,11,15,16)(H2,17,18,19);;;/q;3*+1/p-3/t4-,5-,6-,8-;;;/m1.../s1
InChi Key
QGNCJTNPQREPQC-FCIXCQMASA-K
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $47

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    5 mg

    $116

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    10 mg

    $162

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    Product Description

    5-Bromouridine 5'-triphosphate (5-BrUTP) is a brominated form of UTP that is used to label RNA during transcription. 5-BrUTP in newly-transcribed RNA is then evaluated immunologically with antibodies. This approach has been used to detect or measure RNA transcription with a variety of imaging and molecular methods.1,2,3,4,5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Abe, K.i., Inoue, A., Suzuki, M.G., et al. Global gene silencing is caused by the dissociation of RNA polymerase II from DNA in mouse oocytes. J. Reprod. Dev. 56(5), 502-507 (2010).

    2. Eskiw, C.H., Rapp, A., Carter, D.R.F., et al. RNA polymerase II activity is located on the surface of protein-rich transcription factories. J. Cell Sci. 121(Pt 12), 1999-2007 (2008).

    3. Heinrich, B.S., Cureton, D.K., Rahmeh, A.A., et al. Protein expression redirects vesicular stomatitis virus RNA synthesis to cytoplasmic inclusions. PLoS Pathog. 6(6), 1-14 (2010).

    4. Javed, A., Zaidi, S.K., Gutierrez, S.E., et al. In situ immunofluorescence analysis: Analyzing RNA synthesis by 5-bromouridine-5'-triphosphate labeling. Methods Mol. Biol. 285, 29-31 (2004).

    5. Thiry, M., Cheutin, T., O'Donohue, M.F., et al. Dynamics and three-dimensional localization of ribosomal RNA within the nucleolus. RNA 6(12), 1750-1761 (2000).