A thiol-reactive fluorescent probe
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7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin

Item No. 18209

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
1-[4-[7-(diethylamino)-4-methyl-2-oxo-2H-1-benzopyran-3-yl]phenyl]-1H-pyrrole-2,5-dione
CAS Number
76877-33-3
Molecular Formula
C24H22N2O4
Formula Weight
402.4
Purity
≥95%
Emission
468 nm
Excitation
387 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Slightly solubleMethanol: Slightly soluble
λmax
210, 378 nm
SMILES
CCN(CC)C1=CC=C(C(C)=C(C2=CC=C(N3C(C=CC3=O)=O)C=C2)C(O4)=O)C4=C1
InChi Code
InChI=1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3
InChi Key
YGIABALXNBVHBX-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

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    5 mg

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    10 mg

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    25 mg

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    Product Description

    7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin is a thiol-reactive fluorescent probe.1 It displays excitation/emission maxima of 387/468 nm, respectively, and fluorescence intensity increases when bound to cysteine residues.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sippel, T.O. New fluorochromes for thiols: Maleimide and iodoacetamide derivatives of a 3-phenylcoumarin fluorophore. J. Histochem. Cytochem. 29(2), 314-316 (1981).

    2. Chen, R.F., and Scott, C.H. Atlas of fluorescence spectra and lifetimes of dyes attached to protein. Anal. Lett. 18(4), 393-421 (1985).

    Product Citations

    Aggarwal, G., Zarrow, J.E., Mashhadi, Z., et al. Symmetrically substituted dichlorophenes inhibit N-acyl-phosphatidylethanolamine phospholipase D. The Journal of Biological Chemisty 295(21), 7289-7300 (2020).