A cyanylation reagent for protein cleavage
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Product Type

Biochemicals

Research Area

2-Nitro-5-thiocyanatobenzoic Acid

Item No. 18236

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-nitro-5-thiocyanato-benzoic acid
CAS Number
30211-77-9
Synonyms
  • NTCB
Molecular Formula
C8H4N2O4S
Formula Weight
224.2
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 25 mg/mlDMSO: 11 mg/mlEthanol: 25 mg/mlPBS (pH 7.2): 0.3 mg/ml
λmax
280 nm
SMILES
OC(C1=C([N+]([O-])=O)C=CC(SC#N)=C1)=O
InChi Code
InChI=1S/C8H4N2O4S/c9-4-15-5-1-2-7(10(13)14)6(3-5)8(11)12/h1-3H,(H,11,12)
InChi Key
NQUNIMFHIWQQGJ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    250 mg

    $63

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    1 g

    $134

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    Product Description

    2-Nitro-5-thiocyanatobenzoic acid is used to cyanylate proteins and to specifically cleave the amino-terminal peptide bond of cysteine residues.1,2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Tang, H.Y., and Speicher, D.W. Identification of alternative products and optimization of 2-nitro-5-thiocyanatobenzoic acid cyanylation and cleavage at cysteine residues. Anal. Biochem. 334, 48-61 (2004).

    2. Degani, Y., and Patchornik, A. Cyanylation of sulfhydryl groups by 2-nitro-5-thiocyanobenzoic acid. High-yield modification and cleavage of peptides at cysteine residues. Biochem. 13(1), 1-11 (1974).