A small molecule promotor of hepatocyte differentiation
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Product Categories

Product Type

Biochemicals

Research Area

Cell Biology
Stem Cell Research
Differentiation

BRD-K4477

Item No. 18415

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N,N'-(methylenedi-4,1-phenylene)bis-acetamide
CAS Number
2719-05-3
Synonyms
  • FH1
  • Methylenebis-4,4'-acetanilide
  • NSC 12407
  • Functional Hits 1
Molecular Formula
C17H18N2O2
Formula Weight
282.3
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 2 mg/mlDMSO: 5 mg/mlDMSO:PBS (pH 7.2) (1:1): 0.5 mg/mlEthanol: 0.5 mg/ml
λmax
206, 251 nm
SMILES
CC(NC1=CC=C(CC2=CC=C(NC(C)=O)C=C2)C=C1)=O
InChi Code
InChI=1S/C17H18N2O2/c1-12(20)18-16-7-3-14(4-8-16)11-15-5-9-17(10-6-15)19-13(2)21/h3-10H,11H2,1-2H3,(H,18,20)(H,19,21)
InChi Key
OEXMNSOPAKOPEF-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $54

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    10 mg

    $96

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    25 mg

    $209

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    50 mg

    $367

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    Product Description

    BRD-K4477, also known as functional hits 1 (FH1), is a small molecule promotor of hepatocyte differentiation in vitro.1 It induces maturation of hepatocyte-like cells (iHeps) to a more adult-like liver phenotype in a concentration-dependent manner. BRD-K4477 increases albumin secretion and promotes hepatocyte morphologies including polygonal cell shapes, visible nuclei, and formation of bile canaliculi in iHeps. It also increases expression of ABCB11 and bile-salt export pump (BSEP) mRNA as well as activity of the mature cytochrome P450 (CYP) isoforms CYP3A and CYP2A6 in iHeps. This product is also available as part of Cayman's Hepatocyte Differentiation Reagent Kit (Item No. 44737).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Shan, J., Schwartz, R.E., Ross, N.T., et al. Identification of small molecules for human hepatocyte expansion and iPS differentiation. Nat. Chem. Biol. 9(8), 514-520 (2013).