A chromogenic indicator for NADH
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WST-8

Item No. 18721

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazolium, inner salt, monosodium salt
CAS Number
193149-74-5
Synonyms
  • Water-Soluble Tetrazolium 8
Molecular Formula
C20H13N6O11S2 • Na
Formula Weight
600.5
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMSO: 1 mg/mlPBS (pH 7.2): 5 mg/ml
λmax
255 nm
SMILES
[O-]S(C1=CC(S([O-])(=O)=O)=C(C2=NN(C3=CC=C([N+]([O-])=O)C=C3)[N+](C4=C(OC)C=C([N+]([O-])=O)C=C4)=N2)C=C1)(=O)=O.[Na+]
InChi Code
InChI=1S/C20H14N6O11S2.Na/c1-37-18-10-14(26(29)30)6-9-17(18)24-22-20(21-23(24)12-2-4-13(5-3-12)25(27)28)16-8-7-15(38(31,32)33)11-19(16)39(34,35)36;/h2-11H,1H3,(H-,31,32,33,34,35,36);/q;+1/p-1
InChi Key
VSIVTUIKYVGDCX-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    10 mg

    $37

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    50 mg

    $124

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    100 mg

    $176

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    500 mg

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    Product Description

    WST-8 is a water-soluble tetrazolium salt used for assessing cell metabolic activity. At neutral pH and in the presence of the intermediate electron acceptor, 1-methoxy phenazine methosulfate, NADPH-dependent cellular oxidoreductases, acting via plasma membrane electron transport, reduce the cell-impermeant WST-8 outside the cell to a water-soluble formazan dye with absorbance max at 460 nm.1,2 It is typically used as a cell viability indicator in cell proliferation assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Ukeda, H., Kawana, D., Maeda, S., et al. Spectrophotometric assay for superoxide dismutase based on the reduction of highly water-soluble tetrazolium salts by xanthine-xanthine oxidase. Biosci. Biotechnol. Biochem. 63(3), 485-488 (1999).

    2. Ishiyama, M., Miyazono, Y., Sasamoto, K., et al. A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability. Talanta 44, 1299-1305 (1997).

    Product Citations

    Gordeuk, V.R., Shah, B.N., Zhang, X., et al. The CYB5R3c .350C>G and G6PD A alleles modify severity of anemia in malaria and sickle cell disease. Am. J. Hematol. 95(11), 1269-1279 (2020).