A chromogenic substrate for endoglucanases and cellobiohydrolases
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

4-Nitrophenyl β-D-Cellotrioside

Item No. 19523

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-nitrophenyl O-β-D-glucopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→4)-β-D-glucopyranoside
CAS Number
106927-48-4
Synonyms
  • p-Nitrophenyl β-D-Cellotrioside
Molecular Formula
C24H35NO18
Formula Weight
625.5
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
λmax
221, 296 nm
SMILES
OC[C@H]([C@@H](O[C@@]1([H])[C@H](O)[C@@H](O)[C@H](O[C@]2([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](CO)O1)[C@H](O)[C@H]3O)O[C@H]3OC4=CC=C([N+]([O-])=O)C=C4
InChi Code
InChI=1S/C24H35NO18/c26-5-10-13(29)14(30)17(33)23(39-10)42-21-12(7-28)41-24(19(35)16(21)32)43-20-11(6-27)40-22(18(34)15(20)31)38-9-3-1-8(2-4-9)25(36)37/h1-4,10-24,26-35H,5-7H2/t10-,11-,12-,13-,14+,15-,16-,17-,18-,19-,20-,21-,22-,23+,24+/m1/s1
InChi Key
BETIRLUWOMCBBJ-ZENQEEDISA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    250 µg

    $36

    Add

    500 µg

    $68

    Add

    1 mg

    $108

    Add
    Product Description

    4-Nitrophenyl β-D-cellotrioside is a chromogenic substrate for endoglucanases and cellobiohydrolases.1 Hydrolysis of this substrate by these enzymes liberates 4-nitrophenol, which generates a yellow color that is measured by monitoring absorbance at 405 nm.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hitomi, J., Hatada, Y., Kawaminami, S., et al. Amino acid sequence and stereoselective hydrolytic reaction of an endo-1,4-β-glucanase from a Bacillus strain. Biosci. Biotech. Bioch. 61(12), 2004-2009 (2016).