A potentiometric fluorescent dye
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Di-8-ANEPPS

Item No. 19541

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-[2-[6-(dioctylamino)-2-naphthalenyl]ethenyl]-1-(3-sulfopropyl)-pyridinium, inner salt
CAS Number
157134-53-7
Molecular Formula
C36H52N2O3S
Formula Weight
592.9
Purity
≥98%
Emission
631 nm
Excitation
467 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 2 mg/mlDMSO: 2 mg/mlEthanol: 100 µg/ml
λmax
208, 253, 320, 501 nm
SMILES
[O-]S(CCC[N+](C=C1)=CC=C1/C=C/C2=CC3=CC=C(N(CCCCCCCC)CCCCCCCC)C=C3C=C2)(=O)=O
InChi Code
InChI=1S/C36H52N2O3S/c1-3-5-7-9-11-13-25-38(26-14-12-10-8-6-4-2)36-21-20-34-30-33(18-19-35(34)31-36)17-16-32-22-27-37(28-23-32)24-15-29-42(39,40)41/h16-23,27-28,30-31H,3-15,24-26,29H2,1-2H3
InChi Key
IXFSUSNUALIXLU-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    500 µg

    $54

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    1 mg

    $102

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    5 mg

    $369

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    Product Description

    Di-8-ANEPPS is a potentiometric fluorescent dye that can be used to noninvasively measure transmembrane voltage.1 It is not fluorescent in water, but when bound to the lipid bilayer, becomes strongly fluorescent (ex/em max = ~467/631 nm). The fluorescence intensity of di-8-ANEPPS varies proportionally to changes in transmembrane voltage. This dye is most suitable for measuring larger changes in membrane voltage, such as the onset of induced membrane voltage in nonexcitable cells exposed to external electric fields or action potentials in excitable cells.1 Since di-8-ANEPPS stains the membrane, it can also be used simply as a cell membrane marker.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Pucihar, G., Kotnik, T., and Miklavcic, D. Measuring the induced membrane voltage with Di-8-ANEPPS. J. Vis. Exp. 2009(33), (2016).