A fluorogenic substrate for α-L-iduronidase
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4-Methylumbelliferyl-α-L-Iduronide (free acid)

Item No. 19543

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-methyl-2-oxo-2H-1-benzopyran-7-yl α-L-idopyranosiduronic acid
CAS Number
66966-09-4
Synonyms
  • 4-Methylumbelliferyl-α-L-Idopyranosiduronic Acid
  • 4-MU-α-IdoA
  • MU-α-IdoA
Molecular Formula
C16H16O9
Formula Weight
352.3
Purity
≥90%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMSO: 5 mg/mlEthanol: 1 mg/mlPBS (pH 7.2): 1 mg/ml
λmax
216, 318 nm
SMILES
O=C1C=C(C)C2=C(C=C(O[C@H]3[C@H](O)[C@@H](O)[C@H](O)[C@H](C(O)=O)O3)C=C2)O1
InChi Code
InChI=1S/C16H16O9/c1-6-4-10(17)24-9-5-7(2-3-8(6)9)23-16-13(20)11(18)12(19)14(25-16)15(21)22/h2-5,11-14,16,18-20H,1H3,(H,21,22)/t11-,12-,13+,14+,16+/m0/s1
InChi Key
ARQXEQLMMNGFDU-ZHMBSYLPSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    500 µg

    $72

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    1 mg

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    5 mg

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    Product Description

    4-Methylumbelliferyl-α-L-iduronide (free acid) is a fluorogenic substrate for α-L-iduronidase, an enzyme found in cell lysosomes that is involved in the degradation of glycosaminoglycans such as dermatan sulfate and heparin sulfate. 4-Methylumbelliferyl-α-L-iduronide is cleaved by α-L-iduronidase to release the fluorescent moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases.1 This substrate is used in assays that measure the activity of α-L-iduronidase, which is commonly deficient in a type of lysosomal storage disease called mucopolysaccharidosis.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zhi, H., Wang, J., Wang, S., et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule. J. Spectrosc. 147128 (2013).

    2. Baxter, M.A., Wynn, R.F., Deakin, J.A., et al. Retrovirally mediated correction of bone marrow-derived mesenchymal stem cells from patients with mucopolysaccharidosis type I. Blood 99(5), 1857-1859 (2002).