An amine-reactive fluorescent probe
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Product Categories

Product Type

Biochemicals
Labeling & Detection
Fluorescent Probes

Research Area

Application

Fluorescence

5-Carboxyfluorescein

Item No. 19581

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3',6'-dihydroxy-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid
CAS Number
76823-03-5
Synonyms
  • 5-FAM
Molecular Formula
C21H12O7
Formula Weight
376.3
Purity
≥98%
Emission
518 nm
Excitation
492 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 0.5 mg/mlEthanol: 5 mg/mlEthanol:PBS (pH 7.2) (1:1): 0.5 mg/ml
λmax
209, 225, 277 nm
SMILES
OC1=CC=C(C2(C(C=CC(C(O)=O)=C3)=C3C(O2)=O)C(C=CC(O)=C4)=C4O5)C5=C1
InChi Code
InChI=1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
InChi Key
NJYVEMPWNAYQQN-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    25 mg

    $29

    Add

    50 mg

    $48

    Add

    100 mg

    $77

    Add

    1 g

    $268

    Add
    Product Description

    5-Carboxyfluorescein is a single isomer derivative of 5(6)-carboxyfluorescein (Item No. 17172) that can be used to fluorescently label biomolecules through the interaction of carboxylic acid with primary amines.1 It demonstrates excitation/emission maxima of 492 and 518 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Fischer, R., Mader, O., Jung, G., et al. Extending the applicability of carboxyfluorescein in solid-phase synthesis. Bioconjug. Chem. 14(3), 653-660 (2003).