A fluorescent probe to stain cells
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Product Categories

Product Type

Biochemicals
Labeling & Detection
Fluorescent Probes

Research Area

Application

Flow Cytometry
Fluorescence

5(6)-Carboxyfluorescein diacetate

Item No. 19582

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3',6'-bis(acetyloxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid
CAS Number
124387-19-5
Synonyms
  • CFDA
Molecular Formula
C25H16O9
Formula Weight
460.4
Purity
≥98% (mixture of isomers)
Emission
521 nm
Excitation
494 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 50 mg/mLDMF:PBS (pH 7.2) (1:4): 0.2 mg/mLDMSO: 30 mg/mL
λmax
220, 291 nm
SMILES
O=C(O1)C2=C(C=CC(C(O)=O)=C2)C31C4=CC=C(OC(C)=O)C=C4OC5=C3C=CC(OC(C)=O)=C5.O=C(O6)C7=C(C=C(C(O)=O)C=C7)C86C9=CC=C(OC(C)=O)C=C9OC%10=C8C=CC(OC(C)=O)=C%10
InChi Code
InChI=1S/2C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25;1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h2*3-11H,1-2H3
InChi Key
ZCFZHZUTXKEHLO-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    50 mg

    $63

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    100 mg

    $118

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    250 mg

    $257

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    500 mg

    $481

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    Product Description

    5(6)-Carboxyfluorescein diacetate (CFDA) is a fluorescent probe used to stain cells with intact membranes for analysis in applications such as flow cytometry or fluorescence microscopy. It has relatively slow cellular efflux with a half-life of ~94 minutes and displays fluorescence with excitation/emission maxima of 494/521 nm.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Dive, C., Cox, H., Watson, J.V., et al. Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases. Mol.Cell Probe 2(2), 131-145 (1988).