A cell-permeable fluorescent probe
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Product Categories

Product Type

Biochemicals
Labeling & Detection
Fluorescent Probes

Research Area

Application

Flow Cytometry
Fluorescence

Green CMFDA

Item No. 19583

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3',6'-bis(acetyloxy)-5-(chloromethyl)-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
CAS Number
136832-63-8
Synonyms
  • 5-Chloromethylfluorescein diacetate
Molecular Formula
C25H17ClO7
Formula Weight
464.9
Purity
≥95%
Emission
517 nm
Excitation
492 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
0.1 M Na2CO3: 5 mg/mlDMF: 25 mg/mlDMSO: 30 mg/mlEthanol: 25 mg/mlPBS (pH 7.2): 0.2 mg/ml
SMILES
O=C(O1)C2=C(C=CC(CCl)=C2)C31C4=CC=C(OC(C)=O)C=C4OC5=C3C=CC(OC(C)=O)=C5
InChi Code
InChI=1S/C25H17ClO7/c1-13(27)30-16-4-7-20-22(10-16)32-23-11-17(31-14(2)28)5-8-21(23)25(20)19-6-3-15(12-26)9-18(19)24(29)33-25/h3-11H,12H2,1-2H3
InChi Key
IPJDHSYCSQAODE-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $164

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    5 mg

    $656

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    10 mg

    $1,066

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    Product Description

    Green CMFDA is a cell-permeable fluorescent probe that freely enters living cells, where it is transformed into a cell-impermeable, soluble, fluorescent compound. The resulting molecule does not affect cell viability or proliferation and stably fluoresces for at least 72 hours, allowing for its use in cell tracking.1 It is also transferred to daughter cells. Green CMFDA is brightly fluorescent with standard filters (ex/em max = 492/517 nm) and is often used with additional fluorescent dyes and proteins.2,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Beem, E., and Segal, M.S. Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration. J. Flouresc. 23(5), 975-987 (2013).

    2. Dagley, M.J., Saunders, E.C., Simpson, K.J., et al. High-content assay for measuring intracellular growth of Leishmania in human macrophages. Assay Drug Dev.Technol. 13(7), 389-401 (2015).

    3. Elliott, J.T., Tona, A., and Plant, A.L. Comparison of reagents for shape analysis of fixed cells by automated fluorescence microscopy. Cytometry 52(2), 90-100 (2003).

    Product Citations

    Gonzalez, Z., Carlsen, L., and El-Deiry, W.S. Temozolomide combined with ipilimumab plus nivolumab enhances T cell killing of MGMT-expressing, MSS colorectal cancer cells. Am. J. Cancer Res. 13(1), 216-226 (2023).

    Sun, Y.-J., Chen, Y.-C., Hua, W.-K., et al. Comparison of chimeric antigen receptor-T cell-mediated cytotoxicity assays with suspension tumor cells using plate-based image cytometry method. Cytometry A 103(1), 27-38 (2022).