An amine-containing fluorescent probe
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Product Categories

Product Type

Biochemicals
Labeling & Detection
Fluorescent Probes

Research Area

Application

Fluorescence

Fluorescein-5-thiosemicarbazide

Item No. 19587

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-(3′,6′-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-5-yl)-hydrazinecarbothioamide
CAS Number
76863-28-0
Synonyms
  • FTSC
Molecular Formula
C21H15N3O5S
Formula Weight
421.4
Purity
≥95%
Emission
517 nm
Excitation
495 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 0.5 mg/mlDMSO: 2 mg/mlDMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml
λmax
226, 275 nm
SMILES
OC1=CC=C2C3(C4=CC=C(NC(NN)=S)C=C4C(O3)=O)C5=CC=C(O)C=C5OC2=C1
InChi Code
InChI=1S/C21H15N3O5S/c22-24-20(30)23-10-1-4-14-13(7-10)19(27)29-21(14)15-5-2-11(25)8-17(15)28-18-9-12(26)3-6-16(18)21/h1-9,25-26H,22H2,(H2,23,24,30)
InChi Key
OIMMDIXLTJAVTF-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    25 mg

    $90

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    50 mg

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    Product Description

    Fluorescein-5-thiosemicarbazide (FTSC) is an amine-containing fluorescent probe with excitation/emission maxima of 495/517 nm, respectively.1 It can be reversibly coupled to aldehydes and ketones. FTSC has been used to fluorescently tag diverse molecules, including RNA, saccharides, sialylated glycoproteins, carbonylated proteins, and N-acetylneuraminic acid.1,2,3,4,5,6,7

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zhang, Y., Wang, Z., Zhang, X., et al. One-pot fluorescent labeling of saccharides with fluorescein-5-thiosemicarbazide for imaging polysaccharides transported in living cells. Carbohydr. Res. 346(14), 2156-2164 (2011).

    2. Wu, T.-p., Ruan, K.-c., and Liu, W.-y. A fluorescence-labeling method for sequencing small RNA on polyacrylamide gel. Nucleic Acids Res. 24(17), 3472-3473 (1996).

    3. Mohanty, J.G., Bhamidipaty, S., Evans, M.K., et al. A fluorimetric semi-microplate format assay of protein carbonyls in blood plasma. Anal. Biochem. 400, 289-294 (2010).

    4. Zhang, Y., Yuan, J., Song, J., et al. An efficient method for selectively imaging and quantifying in situ the expression of sialylated glycoproteins on living cells. Glycobiology 23(6), 643-653 (2013).

    5. Havé, M., Leitao, L., Bagard, M., et al. Protein carbonylation during natural leaf senescence in winter wheat, as probed by fluorescein-5-thiosemicarbazide. Plant Biol. (Stuttg) 17(5), 973-979 (2015).

    6. Colombo, G., Clerici, M., Garavaglia, M.E., et al. A step-by-step protocol for assaying protein carbonylation in biological samples. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 1019, (2016).

    7. Dong, R., Li, F., Qin, S., et al. Dataset on inflammatory proteins expressions and sialic acid levels in apolipoprotein E-deficient mice with administration of N-acetylneuraminic acid and/or quercetin. Data Brief 8, (2016).