A fluorogenic substrate for chitinases and chitotriosidases
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

4-Methylumbelliferyl-β-D-N,N',N''-Triacetylchitotrioside

Item No. 19715

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-[[O-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl-(1→4)-O-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl-(1→4)-2-(acetylamino)-2-deoxy-β-D-glucopyranosyl]oxy]-4-methyl-2H-1-benzopyran-2-one
CAS Number
53643-13-3
Synonyms
  • MUF-triNAG
Molecular Formula
C34H47N3O18
Formula Weight
785.8
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 5 mg/mlDMF: 5 mg/mlDMSO: 10 mg/mlDMSO: 10 mg/ml
λmax
317 nm
SMILES
CC(N[C@H]([C@H]1O)[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](CO)[C@H]1O[C@@](O[C@@H]4CO)([H])[C@H](NC(C)=O)[C@@H](O)[C@@H]4O[C@]5([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5NC(C)=O)=O
InChi Code
InChI=1S/C34H47N3O18/c1-12-7-22(44)50-18-8-16(5-6-17(12)18)49-32-24(36-14(3)42)28(47)30(20(10-39)52-32)55-34-25(37-15(4)43)29(48)31(21(11-40)53-34)54-33-23(35-13(2)41)27(46)26(45)19(9-38)51-33/h5-8,19-21,23-34,38-40,45-48H,9-11H2,1-4H3,(H,35,41)(H,36
InChi Key
BNYGKUQXGBVTRE-JFWBNMEYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    500 µg

    $70

    Add

    1 mg

    $96

    Add

    5 mg

    $307

    Add

    10 mg

    $544

    Add
    Product Description

    4-Methylumbelliferyl-β-D-N,N',N''-triacetylchitotrioside is a fluorogenic substrate for chitinases and chitotriosidases.1,2,3 4-Methylumbelliferyl-β-D-N,N',N''-triacetylchitotrioside is cleaved by chitinases and chitotriosidases to release the fluorescent moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases.4 It has been used to screen plasma samples for reduced chitotriosidase activity that may be indicative of lysosomal storage disorders.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Elmonem, M.A., Ramadan, D.I., Issac, M.S., et al. Blood spot versus plasma chitotriosidase: A systematic clinical comparison. Clin. Biochem. 47(1-2), 38-43 (2014).

    2. Howard, M.B., Ekborg, N.A., Taylor, L.E., et al. Genomic analysis and initial characterization of the chitinolytic system of Microbulbifer degradans strain 2-40. J. Bacteriol. 185(11), 3352-3360 (2003).

    3. Mukamolova, G.V., Murzin, A.G., Salina, E.G., et al. Muralytic activity of Micrococcus luteus Rpf and its relationship to physiological activity in promoting bacterial growth and resuscitation. Mol. Microbiol. 59(1), 84-98 (2006).

    4. Zhi, H., Wang, J., Wang, S., et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule. J. Spectrosc. 147128 (2013).