A modifier of protein sulfhydryl groups
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

N-Ethylmaleimide

Item No. 19938

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
1-ethyl-1H-pyrrole-2,5-dione
CAS Number
128-53-0
Synonyms
  • NEM
  • NSC 7638
Molecular Formula
C6H7NO2
Formula Weight
125.1
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
Chloroform: soluble
λmax
218 nm
SMILES
O=C1C=CC(N1CC)=O
InChi Code
InChI=1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3
InChi Key
HDFGOPSGAURCEO-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    5 g

    $54

    Add

    10 g

    $96

    Add

    25 g

    $185

    Add

    50 g

    $341

    Add
    Product Description

    N-Ethylmaleimide is a modifier of sulfhydryl groups in proteins.1,2 It has commonly been used to inactivate cysteine-containing enzymes in vitro. N-Ethylmaleimide inhibits prolyl endopeptidase with an IC50 value of 6.3 µM.3 It alkylates cysteine 519 (Cys519) in voltage-gated potassium channel 7.4 (Kv7.4) and activates Kv7.2 , Kv7.4, and Kv7.5, but not Kv7.3, in CHO cells expressing the human channels when used at a concentration of 50 µM.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kawakita, M., and Yamashita, T. Reactive sulfhydryl groups of sarcoplasmic reticulum ATPase. III. Identification of cysteine residues whose modification with N-ethylmaleimide leads to loss of the Ca2+-transporting activity. J. Biochem. 102(1), 103-109 (1987).

    2. Fatania, H.R., al-Nassar, K.E., and Thomas, N. Chemical modification of rat liver cytosolic NADP+-linked isocitrate dehydrogenase by N-ethylmaleimide. Evidence for essential sulphydryl groups. FEBS Lett. 322(3), 245-248 (1993).

    3. Moriyama, A., Nakanishi, M., and Sasaki, M. Porcine muscle prolyl endopeptidase and its endogenous substrates. J. Biochem. 104(1), 112-117 (1988).

    4. Li, Y., Gamper, N., and Shapiro, M.S. Single-channel analysis of KCNQ K+ channels reveals the mechanism of augmentation by a cysteine-modifying reagent. J. Neurosci. 24(22), 5079-5090 (2004).