Used to label proteins and nucleic acids
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Chemiluminescence

Acridinium NHS ester

Item No. 200200

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
9[[4-[3-[(2,5-dioxo-1-pyrrolidinyl)oxy]-3-oxopropyl]phenoxy]carbonyl]-10-methyl-acridinium, 1,1,1-trifluoromethanesulfonate
CAS Number
177332-37-5
Synonyms
  • Acridinium Compound I
Molecular Formula
C28H23N2O6 • CF3SO3
Formula Weight
632.6
Purity
≥95%
Formulation
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 25 mg/mlDMSO: 25 mg/mlDMSO:PBS (pH 7.2) (1:1): 1 mg/ml
SMILES
C[N+]1=C2C(C=CC=C2)=C(C(OC3=CC=C(CCC(ON4C(CCC4=O)=O)=O)C=C3)=O)C5=C1C=CC=C5.FC(F)(S(=O)([O-])=O)F
InChi Code
InChI=1S/C28H23N2O6.CHF3O3S/c1-29-22-8-4-2-6-20(22)27(21-7-3-5-9-23(21)29)28(34)35-19-13-10-18(11-14-19)12-17-26(33)36-30-24(31)15-16-25(30)32;2-1(3,4)8(5,6)7/h2-11,13-14H,12,15-17H2,1H3;(H,5,6,7)/q+1;/p-1
InChi Key
NFDRKKHKYOEOLR-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $85

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    5 mg

    $314

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    10 mg

    $581

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    50 mg

    $1,243

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    Product Description

    The acridinium NHS ester can be used to label proteins and nucleic acids. The covalently bound acridinium NHS ester will produce chemiluminescence in the presence of hydrogen peroxide. Acridinium-labeled proteins can be used as a detection method in immunoassays.1,2

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Weeks, I., Beheshti, I., McCapra, F., et al. Acridinium esters as high-specific-activity labels in immunoassay. Clin. Chem. 29, 1474-1479 (1983).

    2. Weeks, I., Sturgess, M., Brown, R.C., et al. Immunoassays using acridinium esters. Methods Enzymol. 133, 366-387 (1986).