A fluorescent probe for cell membrane electrical activity
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DISBAC10

Item No. 200280

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
1,3-didecyl-5-[3-(1,3-didecylhexahydro-4,6-dioxo-2-thioxo-5-pyrimidinyl)-2-propenylidene]dihydro-2-thioxo-4,6(1H,5H)-pyrimidinedione
CAS Number
169211-45-4
Molecular Formula
C51H88N4O4S2
Formula Weight
885.4
Purity
≥98%
Emission
560 nm
Excitation
540 nm
Formulation (Request formulation change)
A 10 mg/ml solution in methyl acetate
λmax
541 nm
SMILES
CCCCCCCCCCN1C(O)C(\C=C\C=C2C(=O)N(CCCCCCCCCC)C(=S)N(CCCCCCCCCC)C2=O)C(=O)N(CCCCCCCCCC)C1=S
InChi Code
InChI=1S/C51H90N4O4S2/c1-5-9-13-17-21-25-29-33-40-52-46(56)44(47(57)53(50(52)60)41-34-30-26-22-18-14-10-6-2)38-37-39-45-48(58)54(42-35-31-27-23-19-15-11-7-3)51(61)55(49(45)59)43-36-32-28-24-20-16-12-8-4/h37-39,44,46,56H,5-36,40-43H2,1-4H3/b38-37+
InChi Key
XDRMUCPEPWWVLX-HEFFKOSUSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    1 mg

    $74

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    5 mg

    $327

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    10 mg

    $578

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    Product Description

    DISBAC10 is a voltage-sensitive fluorescent probe used to study cell membrane electrical activity using FRET.1 DISBAC10 displays excitation/emission spectra of 540/560 nm, respectively. In a resting polarized cell, DISBAC10 resides on the outer leaflet of the membrane where it accepts photons from excited fluorescein-labeled proteins and re-emits the photons at a higher wavelength.1 Depolarization of the cell causes rapid translocation of DISBAC10 to the inner leaflet of the membrane, thereby increasing the distance between fluorophores and reducing the FRET signal. DISBAC10 can also be used in conjunction with fluorescein-labeled antibodies or receptor ligands to visualize specific cell classes.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. González, J., and Tsien, R.Y. Voltage sensing by fluorescence resonance energy transfer in single cells. Biophys J. 69(4), 1272-1280 (1995).