A light-generating enzyme
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Product Categories

Product Type

Proteins
Enzymes

Research Area

Application

Chemiluminescence

Luciferase (firefly, recombinant)

Item No. 20398

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
CAS Number
61970-00-1
Purity
≥95% estimated by SDS-PAGE
MW
60 kDa
Formulation (Request formulation change)
A 1 mg/ml solution in 50 mM Tris buffer with 30% glycerol, pH 7.5
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 ml

    $143

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    Product Description

    Luciferase (firefly, recombinant) is a light-generating enzyme that catalyzes an oxidative reaction, using luciferin, ATP, and molecular oxygen to produce oxyluciferin, which yields visible light.1,2 The bioluminescence of this ATP-dependent luciferase is commonly used in reporter systems that are amenable to high-throughput screening.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Baldwin, T.O. Firefly luciferase: The structure is known, but the mystery remains. Structure 4(3), 223-228 (1996).

    2. Xu, T., Close, D., Handagama, W., et al. The expanding toolbox of in vivo bioluminescent Imaging. Front. Oncol. 6, 150 (2016).

    3. Auld, D.S., and Inglese, J. Interferences with luciferase reporter enzymes. Assay Guidance Manual (2016).