A cell-impermeant fluorescent calcium indicator
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Cal Green™ 1 (potassium salt)

Item No. 20399

Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2,2'-((2-(2-(2-(bis(carboxylatomethyl)amino)-5-(3-carboxylato-4-(2,7-dichloro-6-oxido-3-oxo-3H-xanthen-9-yl)benzamido)phenoxy)ethoxy)phenyl)azanediyl)diacetate, hexapotassium salt
Synonyms
  • Calcium Green-1
Molecular Formula
C43H27Cl2N3O16 • 6K
Formula Weight
Purity
≥90%
Emission
531 nm
Excitation
506 nm
A solid
Water: soluble
SMILES
O=C1C(Cl)=CC(C(O2)=C1)=C(C3=CC=C(C(NC4=CC=C(N(CC([O-])=O)CC([O-])=O)C(OCCOC5=C(N(CC([O-])=O)CC([O-])=O)C=CC=C5)=C4)=O)C=C3C([O-])=O)C6=C2C=C([O-])C(Cl)=C6.[K+].[K+].[K+].[K+].[K+].[K+]
InChi Code
InChI=1S/C43H33Cl2N3O16.6K/c44-27-13-25-34(15-31(27)49)64-35-16-32(50)28(45)14-26(35)41(25)23-7-5-21(11-24(23)43(60)61)42(59)46-22-6-8-30(48(19-39(55)56)20-40(57)58)36(12-22)63-10-9-62-33-4-2-1-3-29(33)47(17-37(51)52)18-38(53)54;;;;;;/h1-8,11-16,49H,9-10,17-20H2,(H,46,59)(H,51,52)(H,53,54)(H,55,56)(H,57,58)(H,60,61);;;;;;/q;6*+1/p-6
InChi Key
BLDOQCXFPCZYKG-UHFFFAOYSA-H
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    Product Description

    Cal Green™ 1 is a cell-impermeant fluorescent calcium indicator that is characterized by high quantum yield and low phototoxicity.1 Its peak excitation and emission wavelengths (506 and 531 nm, respectively) are comparable to standard fluorescein dyes, making Cal Green™ 1 appropriate for fluorescent microscopy. Cal Green™ 1 is ~5-fold brighter than fluo-3 (Item Nos. 20403 | 20404) at saturating calcium levels.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Paredes, R.M., Etzler, J.C., Watts, L.T., et alChemical calcium indicators. Methods 46(3), 143-151 (2008).