A fluorescent calcium indicator
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Fluo-3FF (potassium salt)

Item No. 20406

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[2-[2-[2-[bis(carboxymethyl)amino]-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy]ethoxy]-3,4-difluorophenyl]-N-(carboxymethyl)-glycine, pentapotassium salt
CAS Number
348079-14-1
Molecular Formula
C35H21Cl2F2N2O13 • 5K
Formula Weight
981.9
Purity
≥90%
Emission
526 nm
Excitation
506 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Water: soluble
SMILES
O=C([O-])CN(CC([O-])=O)C1=CC=C(C2=C(C=C3Cl)C(OC4=C2C=C(Cl)C([O-])=C4)=CC3=O)C=C1OCCOC5=C(N(CC([O-])=O)CC([O-])=O)C=CC(F)=C5F.[K+].[K+].[K+].[K+].[K+]
InChi Code
InChI=1S/C35H26Cl2F2N2O13.5K/c36-19-8-17-26(10-24(19)42)54-27-11-25(43)20(37)9-18(27)33(17)16-1-3-22(40(12-29(44)45)13-30(46)47)28(7-16)52-5-6-53-35-23(4-2-21(38)34(35)39)41(14-31(48)49)15-32(50)51;;;;;/h1-4,7-11,42H,5-6,12-15H2,(H,44,45)(H,46,47)(H,48,49)(H,50,51);;;;;/q;5*+1/p-5
InChi Key
VRZZNEZQSQRZPU-UHFFFAOYSA-I
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    1 mg

    $423

    Add
    Product Description

    Fluo-3FF is a fluorescent calcium indicator. It is a di-fluorinated analog of Fluo-3 (Item Nos. 20402 | 20403 | 20404) that displays about a 100-fold lower affinity than Fluo-3 for calcium (Kds = 42 and 0.4 µM, respectively).1 Low affinity calcium indicators are particularly useful for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations. Fluo-3FF has excitation/emission maxima of approximately 506 and 526 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. David, G., Barrett, J.N., and Barrett, E.F. Stimulation-induced changes in Ca2+ in lizard motor nerve terminals. J. Physiol. 504(Pt 1), 83-96 (1997).