A ratiometric fluorescent calcium indicator
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Alternative(s)
20419Indo-1 (sodium salt)
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Indo-1 (potassium salt)

Item No. 20418

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-[4-[bis(carboxymethyl)amino]-3-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylic acid, pentapotassium salt
CAS Number
132319-56-3
Molecular Formula
C32H26N3O12 • 5K
Formula Weight
840.1
Purity
≥90%
Emission
475-485 nm (no Ca2+), 400-410 nm (Ca2+-bound)
Excitation
349-364 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Water: Soluble
SMILES
[O-]C(CN(CC([O-])=O)C1=CC=C(C2=CC3=C(C=C(C([O-])=O)C=C3)N2)C=C1OCCOC4=C(N(CC([O-])=O)CC([O-])=O)C=CC(C)=C4)=O.[K+].[K+].[K+].[K+].[K+]
InChi Code
InChI=1S/C32H31N3O12.5K/c1-18-2-6-24(34(14-28(36)37)15-29(38)39)26(10-18)46-8-9-47-27-13-20(5-7-25(27)35(16-30(40)41)17-31(42)43)22-11-19-3-4-21(32(44)45)12-23(19)33-22;;;;;/h2-7,10-13,33H,8-9,14-17H2,1H3,(H,36,37)(H,38,39)(H,40,41)(H,42,43)(H,44,45)
InChi Key
VGZPRMSXVNDMAN-UHFFFAOYSA-I
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $143

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    Product Description

    Indo-1 is a ratiometric fluorescent calcium indicator. It is ideal for analyses using flow cytometry, as it uses a single excitation source, typically 349-364 nm light from an argon-ion laser. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when indo-1 binds calcium.1,2 Indo-1 is prone to photobleaching, which limits its usefulness in methods involving microscopy.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Paredes, R.M., Etzler, J.C., Watts, L.T., et al. Chemical calcium indicators. Methods 46(3), 143-151 (2008).

    2. Grynkiewicz, G., Poenie, M., and Tsien, R.Y. A new generation of Ca2+ indicators with greatly improved fluorescence properties. The Journal of Biological Chemisty 260(6), 3440-3450 (1985).