A very low affinity fluorescent calcium indicator
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Rhod-FF (potassium salt)

Item No. 20443

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2,2'-((2-(2-(6-(bis(carboxylatomethyl)amino)-2,3-difluorophenoxy)ethoxy)-4-(6-(dimethylamino)-3-(dimethyliminio)-3H-xanthen-9-yl)phenyl)azanediyl)diacetate, tripostassium salt
Molecular Formula
C39H35F2N4O11 • 3K
Formula Weight
891.0
Purity
≥90%
Emission
580 nm
Excitation
552 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Water: Soluble
SMILES
CN(C)C(C=C1)=CC2=C1C(C3=CC=C(N(CC([O-])=O)CC([O-])=O)C(OCCOC4=C(N(CC([O-])=O)CC([O-])=O)C=CC(F)=C4F)=C3)=C(C=C/5)C(O2)=CC5=[N+](C)\C.[K+].[K+].[K+]
InChi Code
InChI=1S/C39H38F2N4O11.3K/c1-42(2)23-6-8-25-30(16-23)56-31-17-24(43(3)4)7-9-26(31)37(25)22-5-11-28(44(18-33(46)47)19-34(48)49)32(15-22)54-13-14-55-39-29(12-10-27(40)38(39)41)45(20-35(50)51)21-36(52)53;;;/h5-12,15-17H,13-14,18-21H2,1-4H3,(H3-,46,47,48
InChi Key
ZSOZTLBTBDKAEP-UHFFFAOYSA-K
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    50 µg

    $63

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    Product Description

    Rhod-FF is a difluorinated analog of the cell-impermeant calcium indicator rhod-2. It has a very low affinity for calcium (Kd = 320 µM).1 Low affinity calcium indicators are particularly useful for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations. Rhod-FF has excitation/emission maxima of approximately 552 and 580 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Paredes, R.M., Etzler, J.C., Watts, L.T., et al. Chemical calcium indicators. Methods 46(3), 143-151 (2008).