A cell-permeant precursor of rhod-FF
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Rhod-FF AM

Item No. 20444

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-(9-(4-(bis(2-(acetoxymethoxy)-2-oxoethyl)amino)-3-(2-(6-(bis(2-(acetoxymethoxy)-2-oxoethyl)amino)-2,3-difluorophenoxy)ethoxy)phenyl)-6-(dimethylamino)-3H-xanthen-3-ylidene)-N-methylmethanaminium bromide
Synonyms
  • Rhod-FF Acetoxymethyl ester
Molecular Formula
C51H55F2N4O19 • Br
Formula Weight
1145.9
Purity
≥90%
Emission
580 nm
Excitation
552 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Soluble
SMILES
CN(C)C(C=C1)=CC2=C1C(C3=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC(F)=C4F)=C3)=C(C=C/5)C(O2)=CC5=[N+](C)\C.[Br-]
InChi Code
InChI=1S/C51H55F2N4O19.BrH/c1-30(58)68-26-72-45(62)22-56(23-46(63)73-27-69-31(2)59)40-15-9-34(49-37-12-10-35(54(5)6)20-42(37)76-43-21-36(55(7)8)11-13-38(43)49)19-44(40)66-17-18-67-51-41(16-14-39(52)50(51)53)57(24-47(64)74-28-70-32(3)60)25-48(65)75-29
InChi Key
JAAHQVMOYPWVMY-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    50 µg

    $69

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    Product Description

    Rhod-FF AM is a cell-permeant acetoxymethyl ester of the fluorescent calcium indicator rhod-FF (Item No. 20433). As rhod-FF AM enters cells, it is hydrolyzed by intracellular esterases to produce rhod-FF. Rhod-FF is a difluorinated analog of the cell-impermeant calcium indicator rhod-2. It has a very low affinity for calcium (Kd = 320 µM).1 Low affinity calcium indicators are particularly useful for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations. Rhod-FF has excitation/emission maxima of approximately 552 and 580 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Paredes, R.M., Etzler, J.C., Watts, L.T., et al. Chemical calcium indicators. Methods 46(3), 143-151 (2008).