A spin trap
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TMPO

Item No. 20625

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2,2,4,4-tetramethyl-3,4-dihydro-2H-pyrrole 1-oxide
CAS Number
10135-38-3
Synonyms
  • M4PO
  • NSC 78027
  • 3,3,5,5-Tetramethyl-1-pyrroline N-oxide
Molecular Formula
C8H15NO
Formula Weight
141.2
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Soluble: ≥ 10 mg/mlEthanol: Soluble: ≥ 10 mg/mlPBS (pH 7.2): Soluble: ≥ 10 mg/ml
SMILES
[O-][N+]1=CC(C)(C)CC1(C)C
InChi Code
InChI=1S/C8H15NO/c1-7(2)5-8(3,4)9(10)6-7/h6H,5H2,1-4H3
InChi Key
GUQARRULARNYQZ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    50 mg

    $72

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    100 mg

    $108

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    250 mg

    $251

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    Product Description

    TMPO is a spin trap that reacts with oxygen-, carbon-, and sulfur-centered radicals that has been used in vitro and in vivo.1,2,3,4 It produces longer-lived adducts than DMPO (Item No. 10006436).2 TMPO inhibits thymocyte apoptosis induced by etoposide (Item No. 12092), 6α-methylprednisolone (Item No. 15013), or thapsigargin (Item No. 10522) (IC50s = 30.7, 19.1, and 20.2 mM, respectively).3 It has been used to monitor the formation of hydroxyl radicals in isolated rat hearts following ischemia and reperfusion.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Buettner, G.R., and Britigan, B.E. The spin trapping of superoxide with M4PO (3,3,5,5-Tetramethylpyrroline-N-oxide). Free Radic. Biol. Med. 8(1), 57-60 (1990).

    2. Davies, M.J., Forni, L.G., and Shuter, S.L. Electron spin resonance and pulse radiolysis studies on the spin trapping of sulphur-centered radicals. Chem. Biol. Interact. 61(2), 177-188 (1987).

    3. Slater, A.F., Nobel, C.S., Maellaro, E., et al. Nitrone spin traps and a nitroxide antioxidant inhibit a common pathway of thymocyte apoptosis. Biochem. J. 306(Pt 3)(Pt 3), 771-778 (1995).