A fluorogenic dye
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Rhodamine B isothiocyanate (mixed isomers)

Item No. 20653

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
9-[2-carboxy-4(or 5)-isothiocyanatophenyl]-3,6-bis(diethylamino)-xanthylium, monochloride
CAS Number
944130-99-8
Synonyms
  • RBITC
  • RITC
Molecular Formula
C29H30N3O3S • Cl
Formula Weight
536.1
Purity
Mixture of isomers
Emission
595 nm
Excitation
570 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 25 mg/mlDMF:PBS (pH 7.2)(1:3): 0.1 mg/mlDMSO: 15 mg/mlEthanol: 15 mg/ml
λmax
220, 258, 285, 352, 554 nm
SMILES
CCN(CC)C(C=C1)=CC2=C1C(C3=C(C(O)=O)C=C(N=C=S)C=C3)=C4C(C=C(N(CC)CC)C=C4)=[O+]2.CCN(CC)C(C=C5)=CC6=C5C(C7=C(C(O)=O)C=CC(N=C=S)=C7)=C8C(C=C(N(CC)CC)C=C8)=[O+]6.[Cl-].[Cl-]
InChi Code
InChI=1S/2C29H29N3O3S.2ClH/c1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)25-15-19(30-18-36)9-12-22(25)29(33)34;1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)22-12-9-19(30-18-36)15-25(22)29(33)34;
InChi Key
ZCHFWDRVFPQJSN-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    25 mg

    $72

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    50 mg

    $122

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    100 mg

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    250 mg

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    Product Description

    Rhodamine B isothiocyanate (RITC) is a cell-permeant fluorogenic dye most often used as a conjugate to antibodies and proteins for fluorescence detection in microscopy.1 It displays excitation/emission maxima of 570/595 nm, respectively, and can be used in conjunction with immunohistochemistry for multi-labeling studies.2 It has also been used as an anterograde and retrograde tracer in neurons and in nanoparticles to detect apoptosis.3,4,5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Mao, S.-Y., and Mullins, J.M. Conjugation of fluorochromes to antibodies. Methods Mol. Biol. 588, 43-48 (2010).

    2. Nuschke, A.C., Farrell, S.R., Levesque, J.M., et al. Assessment of retinal ganglion cell damage in glaucomatous optic neuropathy: Axon transport, injury and soma loss. Exp. Eye Res. 141, 111-124 (2015).

    3. Thanos, S., Vidal-Sanz, M., and Aguayo, A.J. The use of rhodamine-B-isothiocyanate (RITC) as an anterograde and retrograde tracer in the adult rat visual system. Brain Res. 406(1-2), 317-321 (1987).

    4. Shi, H., He, X., Wang, K., et al. Rhodamine B isothiocyanate doped silica-coated fluorescent nanoparticles (RBITC-DSFNPs)-based bioprobes conjugated to annexin V for apoptosis detection and imaging. Nanomedicine 3(4), 266-272 (2007).

    5. Jiang, Z., Dong, B., Chen, B., et al. Multifunctional Au@mSiO2/rhodamine B isothiocyanate nanocomposites: Cell imaging, photocontrolled drug release, and photothermal therapy for cancer cells. Small 9(4), 604-612 (2013).