A fluorescent probe that tags proteins in cells
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FlAsH-EDT2

Item No. 20704

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4',5'-bis(1,3,2-dithiarsolan-2-yl)-3',6'-dihydroxy-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
CAS Number
212118-77-9
Synonyms
  • Fluorescein Arsenical Helix Binder
  • Lumio Green
Molecular Formula
C24H18As2O5S4
Formula Weight
664.5
Purity
≥98%
Emission
528 nm
Excitation
508 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 15 mg/mlDMSO: 20 mg/mlDMSO:PBS (pH 7.2) (1:10): 0.09 mg/ml
λmax
515 nm
SMILES
OC1=C([As]2SCCS2)C3=C(C4(C(C=CC=C5)=C5C(O4)=O)C6=CC=C(O)C([As]7SCCS7)=C6O3)C=C1
InChi Code
InChI=1S/C24H18As2O5S4/c27-17-7-5-15-21(19(17)25-32-9-10-33-25)30-22-16(6-8-18(28)20(22)26-34-11-12-35-26)24(15)14-4-2-1-3-13(14)23(29)31-24/h1-8,27-28H,9-12H2
InChi Key
AJNUQUGWNQHQDJ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

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    1 mg

    $47

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    5 mg

    $174

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    10 mg

    $301

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    25 mg

    $462

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    Product Description

    FlAsH-EDT2 is a pro-fluorescent, membrane-permeable biarsenical compound that binds covalently to a tetracysteine sequence (CCPGCC), which is engineered into target proteins.1,2 It binds proteins that have the CCPGCC tag almost immediately after translation.2 FlAsH-EDT2 is commonly used to study protein trafficking, folding, and interactions in living cells or cell lysates.2,3,4 This green-emitting fluorophore is excited at 508 nm, with emission at 528 nm.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Griffin, B.A., Adams, S.R., and Tsien, R.Y. Specific covalent labeling of recombinant protein molecules inside live cells. Science 287(5374), 269-272 (1998).

    2. Rudner, L., Nydegger, S., Coren.L.V., et al. Dynamic fluorescent imaging of human immunodeficiency virus type 1 gag in live cells by biarsenical labeling. J. Virol. 79(7), 4055-4065 (2005).

    3. Luedtke, N.W., Dexter, R.J., Fried, D.B., et al. Surveying polypeptide and protein domain conformation and association with FlAsH and ReAsH. Nat. Chem. Biol. 3(12), 779-784 (2007).

    4. Perdios, L., Bunney.T.D., Warren, S.C., et al. Time-resolved FRET reports FGFR1 dimerization and formation of a complex with its effector PLCγ1. Adv. Biol. Regul. 60, 6-13 (2016).