For the immunochemical detection of phospho-γH2AXSer139
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Histone γH2AX (Phospho-Ser139) Rabbit Monoclonal Antibody (Clone RM224)

Item No. 20721

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • γH2AXS139Ph
Immunogen
A phospho-peptide corresponding to γH2AX (phospho-Ser139)
Clone Designation
RM224
Formulation (Request formulation change)
100 µg protein A affinity-purified antibody from an animal origin–free culture supernatant
Storage Buffer
50% glycerol/PBS with 1% BSA and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, Multiplex, and WB
Cross Reactivity
(+) Phospho-γH2AXSer139(-) Other phosphorylated histones
Species Reactivity
(+) All species
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $500

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    Product Description

    Histone H2AX, a variant of the nucleosome core histone H2A, undergoes phosphorylation on serine 139 in response to DNA damage, particularly when the damage involves formation of double-strand breaks. Phosphorylated H2AX is also termed γH2AX. Induction of γH2AX in cells exposed to genotoxic agents is considered a sensitive and specific reporter of DNA damage.1 This antibody reacts to γH2AX when phosphorylated at Ser139 and does not detect other phosphorylated histones.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Huang, X., Tanaka, T., Kurose, A., et al. Constitutive histone H2AX phosphorylation on Ser-139 in cells untreated by genotoxic agents is cell-cycle phase specific and attenuated by scavenging reactive oxygen species. Int. J. Oncol. 29(2), 495-501 (2006).