For immunochemical detection of 5-mC
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5-Methylcytosine Rabbit Monoclonal Antibody (Clone RM231)

Item No. 20722

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • 5-mC
  • m5C
  • NSC 137776
Immunogen
BSA-conjugated 5-mC
Clone Designation
RM231
Formulation (Request formulation change)
50 µg of protein A-affinity purified antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, Dot blot, FC, ICC, MeDIP
Cross Reactivity
(+) 5-mC in ssDNA and dsDNA(-) 5-hmC(-) Non-methylated cytosine
Species Reactivity
(+) Species-independent
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µg

    $536

    Add
    Cayman Chemical
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    Product Description

    5-Methylcytosine (5-mC) is an epigenetic modification formed from methylation of cytosine by DNA methyltransferases (DNMTs).1 5-mC is an abundant DNA modification that is enriched at CpG dinucleotides. It can be successively oxidized by ten-eleven translocation (TET) dioxygenases to form 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-fC), and 5-carboxylcytosine (5-caC). 5-mC is associated with reduced gene transcription of DNA but is also found in the tRNA, mRNA, and rRNA from bacteria, archaea, and eukaryotes.1,2 Levels of 5-mC on CpG islands in the genes encoding p16, thrombospondin-1 (TSP-1), and methylated in tumor 1 (MINT1) are increased in tumors compared with adjacent normal tissue in patients with renal cell carcinoma.3 Cayman’s 5-Methylcytosine Rabbit Monoclonal Antibody (Clone RM231) can be used for ELISA, dot blot, flow cytometry (FC), immunocytochemistry (ICC), and methylated DNA immunoprecipitation (MeDIP) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Breiling, A., and Lyko, F. Epigenetic regulatory functions of DNA modifications: 5-Methylcytosine and beyond. Epigenetics Chromatin 8, 24 (2015).

    2. Edelheit, S., Schwartz, S., Mumbach, M.R., et al. Transcriptome-wide mapping of 5-methylcytidine RNA modifications in bacteria, archaea, and yeast reveals m5C within archaeal mRNAs. PLoS Genetics 9(6), e1003602 (2013).

    3. Arai, E., Kanai, Y., Ushijima, S., et al. Regional DNA hypermethylation and DNA methyltransferase (DNMT) 1 protein overexpression in both renal tumors and corresponding nontumorous renal tissues. Int. J. Cancer 119(2), 288-296 (2006).