For immunochemical detection of 5-hmC
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5-Hydroxymethylcytosine Rabbit Monoclonal Antibody (Clone RM236)

Item No. 20723

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • 5-hmC
Immunogen
BSA-conjugated 5-hydroxy methylcytosine
Clone Designation
RM236
Formulation (Request formulation change)
50 µg of protein A-affinity purified antibody from an animal origin–free culture supernatant from an animal origin–free culture supernatant
Storage Buffer
50% glycerol/PBS, with 1% BSA and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
Dot blot, ELISA, hMeDIP, ICC, IHC
Cross Reactivity
(+) 5-Hydroxymethylcytosine in both single-stranded and double-stranded DNA(-) Non-methylated cytosine(-) Methylcytosine in DNA
Species Reactivity
(+) All species
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    50 µg

    $536

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    Cayman Chemical
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    Product Description

    5-Hydroxymethylcytosine (5-hmC) is an epigenetic modification formed from the oxidation of 5-methylcytosine by Tet dioxygenases.1 5-hmC is primarily a stable DNA modification, but it can be oxidized by Tet enzymes and its products further modified to generate nonmethylated cytosine, indicating a role as an intermediate in DNA demethylation as well.2,3,4 It is associated with actively transcribed genes and recognized by a variety of proteins, including proteins involved in DNA repair and DNA metabolic processes.2,4 5-hmC has been found in all mammalian tissues but levels are higher in the brain relative to other tissues.5 The percentage of genomic 5-hmC in mouse cerebellum increases during postnatal development until adulthood, and genes acquiring intragenic 5-hmC are enriched in pathways associated with age-related neurodegenerative disease pathways in adult mice.6 In contrast, 5-hmC levels are reduced by up to 8-fold in cancer tissues.2,7 Cayman’s 5-Hydroxymethylcytosine Rabbit Monoclonal Antibody (Clone RM236) can be used for dot blot, ELISA, hydroxymethylated DNA immunoprecipitation (hMeDIP), immunocytochemistry (ICC), and immunohistochemistry (IHC) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Tahiliani, M., Koh, K.P., Shen, Y., et al. Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1. Science 324(5929), 930-935 (2009).

    2. Bachman, M., Uribe-Lewis, S., Yang, X., et al. 5-Hydroxymethylcytosine is a predominantly stable DNA modification. Nat. Chem. 6(12), 1049-1055 (2014).

    3. Breiling, A., and Lyko, F. Epigenetic regulatory functions of DNA modifications: 5-Methylcytosine and beyond. Epigenetics Chromatin 8, 24 (2015).

    4. Spruijt, C.G., Gnerlich, F., Smits, A.H., et al. Dynamic readers for 5-(hydroxy)methylcytosine and its oxidized derivatives. Cell 152(5), 1146-1159 (2013).

    5. Globisch, D., Münzel, M., Müller, M., et al. Tissue distribution of 5-hydroxymethylcytosine and search for active demethylation intermediates. PLoS One 5(12), e15367 (2010).

    6. Song, C.X., Szulwach, K.E., Fu, Y., et al. Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine. Nat. Biotechnol. 29(1), 68-72 (2011).

    7. Wang, Z., Du, M., Yuan, Q., et al. Epigenomic analysis of 5-hydroxymethylcytosine (5hmC) reveals novel DNA methylation markers for lung cancers. Neoplasia 22(3), 154-161 (2020).