An antagonist of P2X1, P2X3, and P2X2/3 receptors and a fluorescent probe
Related Products
Alternative(s)
38492TNP-ATP (sodium salt)
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

TNP-ATP (triethylammonium salt)

Item No. 20902

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
((3a'R,4'R,6'R,6a'R)-4'-(6-amino-9H-purin-9-yl)-6'-(((((((hydroxyoxidophosphoryl)oxy)oxidophosphoryl)oxy)oxidophosphoryl)oxy)methyl)-2,6-dinitro-3a',4',6',6a'-tetrahydrospiro[cyclohexane-1,2'-furo[3,4-d][1,3]dioxole]-2,5-dien-4-ylidene)azinate, triethyltetraammonium salt
Molecular Formula
C16H13N8O19P3 • 4C6H16N
Formula Weight
1123.0
Purity
≥95%
Emission
547 nm
Excitation
403 nm
Formulation (Request formulation change)
A 10 mM solution in water
Solubility(Learn about Variance in Solubility)
Water: Soluble
SMILES
O=P(OP(OP(O)([O-])=O)([O-])=O)([O-])OC[C@@H]1[C@]2([H])[C@](OC3(C([N+]([O-])=O)=C/C(C=C3[N+]([O-])=O)=[N+]([O-])/[O-])O2)([H])[C@H](N4C=NC5=C4N=CN=C5N)O1.CC[NH+](CC)CC.CC[NH+](CC)CC.CC[NH+](CC)CC.CC[NH+](CC)CC
InChi Code
InChI=1S/C16H16N8O19P3.4C6H15N/c17-13-10-14(19-4-18-13)21(5-20-10)15-12-11(7(39-15)3-38-45(34,35)43-46(36,37)42-44(31,32)33)40-16(41-12)8(23(27)28)1-6(22(25)26)2-9(16)24(29)30;4*1-4-7(5-2)6-3/h1-2,4-5,7,11-12,15H,3H2,(H6-,17,18,19,25,26,31,32,33,34,3
InChi Key
XTSNRUFLZVGKOU-NRBYJGOSSA-O
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    5 mg

    $493

    Add
    Product Description

    TNP-ATP is a derivative of ATP and an antagonist at the purinergic receptor subtypes P2X1, P2X3, and P2X2/3 (IC50s = 6, 0.9, and 7 nM, respectively).1 It is selective for those receptor subtypes over P2X2, P2X4, and P2X7 receptors (IC50s = 2,000, 15,200, and >30,000 nM, respectively). TNP-ATP inhibits calcium flux in 1321N1 cells expressing P2X3 and P2X2/3 receptors (IC50s = 10 and 62 nM, respectively).2 In a mouse model of visceral pain, TNP-ATP reduces acetic-acid induced writhing with an ED50 value of 6.35 µmol/kg. TNP-ATP is also a fluorescent probe for the activity of ATP-binding enzymes, such as insulin-degrading enzyme (IDE).3 It displays excitation/emission maxima of 403 and 547 nm, respectively, with a four-fold increase in fluorescence intensity and an emission shift to 538 nm when bound to IDE.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Virginio, C., Robertson, G., Surprenant, A., et al. Trinitrophenyl-substituted nucleotides are potent antagonists selective for P2X1, P2X3, and heteromeric P2X2/3 receptors. Mol. Pharmacol. 53(6), 969-973 (1998).

    2. Honore, P., Mikusa, J., Bianchi, B., et al. TNP-ATP, a potent P2X3 receptor antagonist, blocks acetic acid-induced abdominal constriction in mice: Comparison with reference analgesics. Pain 96(1-2), 99-105 (2002).

    3. Yao, H., and Hersh, L.B. Characterization of the binding of the fluorescent ATP analog TNP-ATP to insulysin. Arch. Biochem. Biophys. 451(2), 175-181 (2006).