A fluorogenic substrate of GALNS
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4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate (sodium salt)

Item No. 20907

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-methyl-7-[(6-O-sulfo-β-D-galactopyranosyl)oxy]-2H-1-benzopyran-2-one, monosodium salt
CAS Number
206443-06-3
Synonyms
  • 4-MU β-D-galactopyranoside-6-sulfate
  • 4-Methylumbelliferyl Gal-6S
  • 4-MU-Gal-6S
Molecular Formula
C16H17O11S • Na
Formula Weight
440.4
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
318 nm
SMILES
O=C(O1)C=C(C)C(C1=C2)=CC=C2O[C@@H]3O[C@H](COS([O-])(=O)=O)[C@H](O)[C@H](O)[C@H]3O.[Na+]
InChi Code
InChI=1S/C16H18O11S.Na/c1-7-4-12(17)26-10-5-8(2-3-9(7)10)25-16-15(20)14(19)13(18)11(27-16)6-24-28(21,22)23;/h2-5,11,13-16,18-20H,6H2,1H3,(H,21,22,23);/q;+1/p-1/t11-,13+,14+,15-,16-;/m1./s1
InChi Key
KHTOBGQRIASILC-XNSOHGFJSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $104

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    5 mg

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    10 mg

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    Product Description

    4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate (sodium salt) (4-MU-Gal-6S) is a fluorogenic substrate used to quantify N-acetylgalactosamine-6-sulphatase (GALNS) activity.1 4-MU-Gal-6S is cleaved by GALNS to release the fluorescent moiety 4-MU. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases.2 It has been used to detect Morquio disease type A, a lysosomal storage disorder in which GALNS is deficient.1 4-MU-Gal-6S can be used to assess GALNS activity in a very small blood volume to determine the extent of deficiency.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Van Diggelen, O.P., Zhao, H., Kleijer, W.J., et al. A fluorimetric enzyme assay for the diagnosis of Morquio disease type A (MPS IV A). Clin. Chim. Acta 187(2), 131-139 (1990).

    2. Zhi, H., Wang, J., Wang, S., et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule. J. Spectrosc. 147128 (2013).

    3. Cozma, C., Eichler, S., Wittmann, G., et al. Diagnosis of Morquio syndrome in dried blood spots based on a new MRM-MS assay. PLoS One 10(7), (2015).