A fluorescent compound
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N-Butylfluorescein

Item No. 20909

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3'-butoxy-6'-hydroxy-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
CAS Number
335193-91-4
Molecular Formula
C24H20O5
Formula Weight
388.4
Purity
≥98%
Emission
512 nm
Excitation
467 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
Chloroform: Slightly solubleMethanol: Slightly soluble
λmax
226, 277 nm
SMILES
OC1=CC=C(C2(C(C=CC=C3)=C3C(O2)=O)C(C=CC(OCCCC)=C4)=C4O5)C5=C1
InChi Code
InChI=1S/C24H20O5/c1-2-3-12-27-16-9-11-20-22(14-16)28-21-13-15(25)8-10-19(21)24(20)18-7-5-4-6-17(18)23(26)29-24/h4-11,13-14,25H,2-3,12H2,1H3
InChi Key
UMLSFGLXYOSGJH-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 mg

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    5 mg

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    10 mg

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    Product Description

    N-Butylfluorescein is a fluorescent compound that displays excitation/emission maxima of 467/512 nm, respectively.1 It has been used in the synthesis of butyl fluorescein myo-inositol phosphate (butyl FLIP), a fluorogenic substrate of phosphatidylinositol-specific phospholipase C (PI-PLC).2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Li, X., and Taylor, J.S. General strategy for the preparation of membrane permeable fluorogenic peptide ester conjugates for in vivo studies of ester prodrug stability. Bioorg. Med. Chem. 12(3), 545-552 (2004).

    2. Zaikova, T.O., Rukavishnikov, A.V., Birrell, G.B., et al. Synthesis of fluorogenic substrates for continuous assay of phosphatidylinositol-specific phospholipase C. Bioconjug. Chem. 12(2), 307-313 (2001).